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Microdissecção e captura a laser na investigação do gene TP53 em tecidos incluídos em parafina
(2007-02-01)
Background: Laser-capture micro-dissection (LCM) is a recently developed procedure that provides single cells or specific cell groups for molecular analysis. Currently, there are few LCM systems in Brazil, in such a way ...
Expression of the sigma35 and cry2AB genes involved in Bacillus thuringiensis virulence
(Universidade de São Paulo (USP), Escola Superior de Agricultura Luiz de Queiroz (ESALQ), 2014)
Report of a chimeric origin of transposable elements in a bovine-coding gene
(2008-04-22)
Despite the wide distribution of transposable elements (TEs) in mammalian genomes, part of their evolutionary significance remains to be discovered. Today there is a substantial amount of evidence showing that TEs are ...
Diversity of cry genes and genetic characterization of Bacillus thuringiensis isolated from Brazil
(2004-08-01)
Two hundred and eighteen Bacillus thuringiensis isolates from Brazil were characterized by the presence of crystal protein genes by PCR with primers specific to different cry and cyt genes. Among these isolates, 95 were ...
Selection and validation of reference genes for quantitative gene expression analyses in various tissues and seeds at different developmental stages in Bixa orellana L.
(Physiology and Molecular Biology of Plants, 2018)
Avaliação dos polimorfismos nos genes TP53, P27 e FAS em mulheres com endometriose
(Universidade Federal de São Paulo (UNIFESP), 2009-08-26)
Introduction: Endometriosis is a benign gynaecologycal disease, defined as the presence of functional endometrial implants out of uterus, mainly in pelvis. Cell cycle and apoptosis disturbs could be associated with this ...
Synthetic and natural polycations for gene therapy: State of the art and new perspectives
(Bentham Science Publ Ltd, 2006-02-01)
Currently, the major drawback of gene therapy is the gene transfection rate. The two main types of vectors that. are used in gene therapy are based on viral or non-viral gene delivery systems. There are several non-viral ...
Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp citri during infection of Citrus sinensis
(Springer, 2011-06-01)
Xanthomonas citri subsp. citri (Xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (Citrus sinensis (L.) Osbeck). The expression of nine candidate internal reference genes was analyzed in Xcc grown ...
Diversity of cry genes and genetic characterization of Bacillus thuringiensis isolated from Brazil
(2004-08-01)
Two hundred and eighteen Bacillus thuringiensis isolates from Brazil were characterized by the presence of crystal protein genes by PCR with primers specific to different cry and cyt genes. Among these isolates, 95 were ...