Buscar
Mostrando ítems 11-20 de 1033
Interaction of a synthetic antimicrobial peptide with model membrane by fluorescence spectroscopy
(Springer, 2013-12-01)
Static and time-resolved fluorescence of tryptophan and ortho-aminobenzoic acid was used to investigate the interaction of the synthetic antimicrobial peptide L1A (IDGLKAIWKKVADLLKNT-NH2) with POPC and POPC:POPG. N-acetylated ...
Agonist mobility on supported lipid bilayers affects Fas mediated death response
(Elsevier Science, 2015-11-30)
Extrinsic apoptosis is initiated by recognition and clustering of the single-pass transmembrane proteins Fas ligand and Fas expressed at the surface of closely apposed lymphocytes and target cells, respectively. Since ...
Ortho-aminobenzoic acid-labeled bradykinins in interaction with lipid vesicles: Fluorescence study
(Wiley-Blackwell, 2002-12-05)
The peptide hormone bradykinin (BK) (Arg(1)-Pro(2)-Pro(3)-Gly(4)-Phe(5)-Ser(6)-Pro(7)-Phe(8)-Arg(9)) and its shorter homolog BK1-5 (Arg(1)-Pro(2)-Pro(3)-Gly(4)-Phe(5)) were labeled with the extrinsic fluorescent probe ...
Kinetics of lipid-membrane binding and conformational change of L-BABP
(Academic Press Inc Elsevier Science, 2009-12)
We designed an experimental approach to differentiate the kinetics of protein binding to a lipid membrane from the kinetics of the associated conformational change in the protein. We measured the fluorescence intensity of ...
Fluorescent redox-dependent labeling of lipid droplets in cultured cells by reduced phenazine methosulfate
(Elsevier, 2020-06)
Natural and synthetic phenazines are widely used in biomedical sciences. In dehydrogenase histochemistry, phenazine methosulfate (PMS) is applied as a redox reagent for coupling reduced coenzymes to the reduction of ...
Alterations in chlorophyll a fluorescence, pigment concentrations and lipid peroxidation to chilling temperature in coffee seedlings
(2009-11-01)
Coffea arabica L. is considered to be sensitive to low temperatures throughout its life cycle. In some Brazilian regions, seedling production occurs under shade conditions and during the winter, with average temperatures ...
Alterations in chlorophyll a fluorescence, pigment concentrations and lipid peroxidation to chilling temperature in coffee seedlings
(2009-11-01)
Coffea arabica L. is considered to be sensitive to low temperatures throughout its life cycle. In some Brazilian regions, seedling production occurs under shade conditions and during the winter, with average temperatures ...
Resolution of complex fluorescence spectra of lipids and nicotinic acetylcholine receptor by multivariate analysis reveals protein-mediated effects on the receptor's immediate lipid microenvironment
(Springer, 2008-12)
Analysis of fluorescent spectra from comp lex biological systems containing various fluorescent probes with overlapping emissi on bands is a challenging task. Valuable information can be extracted from the full spectra, ...
Giant vesicles, laurdan, and two-photon fluorescence microscopy: Evidence of lipid lateral separation in bilayers
(Elsevier Academic Press Inc., 2003-03)
This chapter describes giant vesicles, Laurdan, and two-photon fluorescence microscopy. The combination of Laurdan, giant unilamellar vesicles (GUVs), and two-photon fluorescence microscopy has been extremely useful in ...