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Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression
(Bioscientifica, 2015-07)
The aim of this study was to evaluate the capacity of domestic cat (Dc, Felis silvestris) oocytes to reprogram the nucleus of cheetah (Ch, Acinonyx jubatus) cells by interspecies SCNT (iSCNT), by using embryo aggregation. ...
State of the art on animal embryonic chimeras
(2013)
Embryonic chimerism is generally used in basic research and in vivo diagnosis of undifferentiated embryonic stem cells (ESC), mostly using mice embryos, although there have been reports in the literature on using rat, ...
In Vitro Development and Cell Allocation After Aggregation of Syngeneic Wild Type and Fluorescence-Expressing Bovine Cloned Embryos
(UNIV FED RIO GRANDE DO SULPORTO ALEGRE RS, 2012)
Background: The in vitro production (IVP) of embryos by in vitro fertilization or cloning procedures has been known to cause epigenetic changes in the conceptus that in turn are associated with abnormalities in pre- and ...
Screening of biotechnical parameters for production of bovine inter-subspecies embryonic chimeras by the aggregation of tetraploid Bos indicus and diploid crossbred Bos taurus embryos
(2016-03-01)
The aggregation of a tetraploid zebu embryo (Bos indicus, a thermotolerant breed) with a diploid taurine embryo (Bos taurus, a thermosensitive breed) should create a complete taurine fetus, whose extra-embryonic components, ...
In vitro development of cloned bovine embryos produced by handmade cloning using somatic cells from distinct levels of cell culture confluence
(FUNPEC-EDITORA, 2010)
The relationship between the level of cell confluence near the plateau phase of growth and blastocyst yield following somatic cell cloning is not well understood. We examined the effect of distinct cell culture confluence ...
Aggregation of Leopardus geoffroyi hybrid embryos with domestic cat tetraploid blastomeres
(BioScientifica, 2021-05)
Heterospecific embryo transfer of an endangered species has been carried out using recipients from related domestic females. Aggregation of an embryo from an endangered species with a tetraploid embryo from the species to ...
Developmental Potential of Bovine Hand-Made Clone Embryos Reconstructed by Aggregation or Fusion with Distinct Cytoplasmic Volumes
(MARY ANN LIEBERT INC, 2009)
Animal cloning has been associated with developmental abnormalities, with the level of heteroplasmy caused by the procedure being one of its potential limiting factors. The aim of this study was to determine the effect of ...
CHIMERISM BY AGGREGATION of BISECTED IN VITRO PRODUCED BOVINE EMBRYOS
(CSIRO Publishing, 2011-01-01)