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New approaches regarding the in vitro maturation of oocytes: Manipulating cyclic nucleotides and their partners in crime
(2017-01-01)
Several discoveries have been described recently (5-10 years) about the biology of ovarian follicles (oocyte, cumulus cells and granulosa cells), including new aspects of cellular communication, the control of oocyte ...
Supplementing in vitro embryo production media by NPPC and sildenafil affect the cytoplasmic lipid content and gene expression of bovine cumulus-oocyte complexes and embryos
(2018-03-01)
In our study, we added natriuretic peptide type C (NPPC) and/or sildenafil during in vitro maturation (IVM) of bovine cumulus-oocyte complexes (COCs) followed by in vitro culture (IVC) of embryos with or without sildenafil. ...
Efeito de um meio condicionado por células do Cumulus oophorus de bovinos na maturação e no potencial de desenvolvimento embrionário in vitro de oócitos desnudos
(Universidade Federal de Minas GeraisUFMG, 2017-08-31)
One of the challenges of reproductive biology and medicine is to understand the nature of the molecular and cellular processes that control oocyte developmental competence. This competence, acquired through maturation, a ...
Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation
(Elsevier Science, 2016-07)
Hormonal ovarian stimulation may affect transcripts in somatic cells of cumulus-oocyte complexes (COCs) and affect the resulting oocyte quality. Here, in parallel with morphological classification and in vitro maturation ...
Analyses of apoptosis and DNA damage in bovine cumulus cells after in vitro maturation with different copper concentrations: consequences on early embryo development
(Cambridge University Press, 2016-11)
The aim of this study was to investigate the influence of copper (Cu) during in vitro maturation (IVM) on apoptosis and DNA integrity of cumulus cells (CC); and oocyte viability. Also, the role of CC in the transport of ...