Buscar
Mostrando ítems 1-10 de 21
Equine cloning: In vitro and In vivo development of aggregated embryos
(Society For The Study Of Reproduction, 2012-07)
The production of cloned equine embryos remains highly inefficient. Embryo aggregation has not yet been tested in the equine, and it might represent an interesting strategy to improve embryo development. This study evaluated ...
Embryo Aggregation in Pig Improves Cloning Efficiency and Embryo Quality
(Public Library of Science, 2016-02)
In this study, we analyzed the effects of the cloned embryo aggregation on in vitro embryo development and embryo quality by measuring blastocyst diameter and cell number, DNA fragmentation levels and the expression of ...
The aggregation of four reconstructed zygotes is the limit to improve the developmental competence of cloned equine embryos
(Public Library Of Science, 2014-11)
Embryo aggregation has been demonstrated to improve cloning efficiency in mammals. However, since no more than three embryos have been used for aggregation, the effect of using a larger number of cloned zygotes is unknown. ...
In Vitro Development and Cell Allocation After Aggregation of Syngeneic Wild Type and Fluorescence-Expressing Bovine Cloned Embryos
(UNIV FED RIO GRANDE DO SULPORTO ALEGRE RS, 2012)
Background: The in vitro production (IVP) of embryos by in vitro fertilization or cloning procedures has been known to cause epigenetic changes in the conceptus that in turn are associated with abnormalities in pre- and ...
Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression
(Bioscientifica, 2015-07)
The aim of this study was to evaluate the capacity of domestic cat (Dc, Felis silvestris) oocytes to reprogram the nucleus of cheetah (Ch, Acinonyx jubatus) cells by interspecies SCNT (iSCNT), by using embryo aggregation. ...
6 efficient transgenesis in bovine embryos by fertilization with androgenetic transgenic blastomeres
(Csiro Publishing, 2010-01)
Pronuclear microinjection and intracytoplasmic sperm injection-mediated gene transfer (ICSI-mgt) are useful techniques to obtain transgenic animals. Nevertheless, a high frequency of mosaic expression is observed in embryos ...
Developmental Potential of Bovine Hand-Made Clone Embryos Reconstructed by Aggregation or Fusion with Distinct Cytoplasmic Volumes
(MARY ANN LIEBERT INC, 2009)
Animal cloning has been associated with developmental abnormalities, with the level of heteroplasmy caused by the procedure being one of its potential limiting factors. The aim of this study was to determine the effect of ...
Effects of fetuin on zona pellucida hardening, fertilization and embryo development in cattle
(Elsevier B.V., 2002-06-17)
Mammalian oocytes can undergo spontaneous meiotic maturation when they are liberated from their follicles and cultured in vitro; however, the zona pellucida (ZP) becomes resistant to chymotrypsin digestion, or hardens, ...
Effects of fetuin on zona pellucida hardening, fertilization and embryo development in cattle
(Elsevier B.V., 2002-06-17)
Mammalian oocytes can undergo spontaneous meiotic maturation when they are liberated from their follicles and cultured in vitro; however, the zona pellucida (ZP) becomes resistant to chymotrypsin digestion, or hardens, ...