Otro
Microbiological Assay for the Determination of Colistin Sulphate
Registro en:
Current Analytical Chemistry. Sharjah: Bentham Science Publ Ltd, v. 10, n. 3, p. 443-448, 2014.
1573-4110
WOS:000337253600016
Autor
Calixto, Giovana
Domingos, Luciana Elisete
Fabri, Nathalie Thomazini
Carmo, Thais Adriana do
Moreno, Andreia de Haro
Salgado, Hérida Regina Nunes
Chorilli, Marlus
Resumen
An innovative microbiological assay was developed for the determination of colistin sulphate at various concentrations (192-469 IU/mL); using Bordetella bronchiseptica ATCC 4617 as microorganism test. This assay revealed subtle changes not demonstrated by chemical methods, such as chemical degradation and low of potency, but by evaluating the potency of colistin, which is very important for the analysis of antibiotics. The method was validated according to ICH guidelines, proving to be linear ( r(2) = 0.9985), precise ( CV = 1.32%) and accurate ( 100.9 +/- 1.33%). It was concluded that the microbiological assay is satisfactory for in vitro quantitation of the antibacterial activity of colistin sulphate. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)