Otro
Effect of phosphoric acid on the degradation of human dentin matrix
Registro en:
Journal of Dental Research, v. 92, n. 1, p. 87-91, 2013.
0022-0345
1544-0591
10.1177/0022034512466264
WOS:000312209700015
2-s2.0-84870925270
Autor
Tezvergil-Mutluay, A.
Mutluay, M.
Seseogullari-Dirihan, R.
Agee, K. A.
Key, W. O.
Scheffel, D. L S
Breschi, L.
Mazzoni, A.
Tjäderhane, L.
Nishitani, Y.
Tay, F. R.
Pashley, D. H.
Resumen
This study determined if dentin proteases are denatured by phosphoric acid (PA) used in etch-and-rinse dentin adhesives. Dentin beams were completely demineralized with EDTA for 30 days. We acid-etched experimental groups by exposing the demineralized dentin beams to 1, 10, or 37 mass% PA for 15 sec or 15 min. Control beams were not exposed to PA but were incubated in simulated body fluid for 3 days to assay their total endogenous telopeptidase activity, by their ability to solubilize C-terminal crosslinked telopeptides ICTP and CTX from insoluble dentin collagen. Control beams released 6.1 ± 0.8 ng ICTP and 0.6 ± 0.1 ng CTX/mg dry-wt/3 days. Positive control beams pre-incubated in p-aminophenylmercuric acetate, a compound known to activate proMMPs, released about the same amount of ICTP peptides, but released significantly less CTX. Beams immersed in 1, 10, or 37 mass% PA for 15 sec or 15 min released amounts of ICTP and CTX similar to that released by the controls (p > 0.05). Beams incubated in galardin, an MMP inhibitor, or E-64, a cathepsin inhibitor, blocked most of the release of ICTP and CTX, respectively. It is concluded that PA does not denature endogenous MMP and cathepsin activities of dentin matrices. © 2013 International & American Associations for Dental Research.
Materias
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Effect of phosphoric acid on the degradation of human dentin matrix
University of Turku; Georgia Health Sciences University; Universidade Estadual Paulista (Unesp); University of Trieste; University of Bologna; Oulu University Hospital; Graduate School of Medicine, Dentistry and Pharmaceutical Sciences; Unit of Bologna C/o IOR (2013-01-01)This study determined if dentin proteases are denatured by phosphoric acid (PA) used in etch-and-rinse dentin adhesives. Dentin beams were completely demineralized with EDTA for 30 days. We acid-etched experimental groups ... -
Effect of phosphoric acid on the degradation of human dentin matrix
University of Turku; Georgia Health Sciences University; Universidade Estadual Paulista (Unesp); University of Trieste; University of Bologna; Oulu University Hospital; Graduate School of Medicine, Dentistry and Pharmaceutical Sciences; Unit of Bologna C/o IOR (2013-01-01)This study determined if dentin proteases are denatured by phosphoric acid (PA) used in etch-and-rinse dentin adhesives. Dentin beams were completely demineralized with EDTA for 30 days. We acid-etched experimental groups ... -
Structural and biochemical alterations during the healing process of tendons treated with Aloe vera
Aro, AA; Nishan, U; Perez, MO; Rodrigues, RA; Foglio, MA; Carvalho, JE; Gomes, L; Vidal, BC; Pimentel, ER