Two Digestive Trypsins Occur in Three Species of Neotropical Anophelines
Journal of Medical Entomology, v. 40, n. 6, p. 991-995, 2003.
Caroci, A. S.
Ribolla, P. E.
De Bianchi, A. G.
Trypsin activity increases in the midgut of Anopheles aquasalis, Anopheles albitarsis, and Anopheles darlingi after a bloodmeal. The activity returns to basal levels at the time the blood is completely digested. Affinity chromatography, reversed-phase high performance liquid chromatography (HPLC), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to sequentially purify the mosquito trypsins found in the midguts at 24 h after feeding. Amino-terminal sequencing of the purified trypsins showed the occurrence of two distinct trypsins in the midgut of each of the mosquitoes studied. The sequences obtained are similar to those of the trypsins of other hematophagous insects.
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Miziara, Melissa Nunes; Riggs, Penny K.; Amaral, M. Elisabete J.
Universidade Estadual Paulista (UNESP); Research Division (2004-12-01)Genomic sequence comparison across species has enabled the elucidation of important coding and regulatory sequences encoded within DNA. Of particular interest are the noncoding regulatory sequences, which influence gene ...
Universidade de São Paulo (USP); University of California; Universidade Estadual Paulista (UNESP) (2003-11-01)Trypsin activity increases in the midgut of Anopheles aquasalis, Anopheles albitarsis, and Anopheles darlingi after a bloodmeal. The activity returns to basal levels at the time the blood is completely digested. Affinity ...