Otro
Quantitative determination of maitenin and 22 beta-hydroxymaitenin in callus of Maytenus aquifolium (Celastraceae) by reverse phase high performance liquid chromatography
Registro en:
Phytochemical Analysis. Hoboken: Wiley-blackwell, v. 9, n. 5, p. 245-247, 1998.
0958-0344
10.1002/(SICI)1099-1565(199809/10)9:5<245
WOS:000076133700006
Autor
Corsino, J.
Alecio, A. C.
Ribeiro, M. L.
Furlan, Maysa
Pereira, AMS
Duarte, I. B.
Franca, S. C.
Resumen
Explants of Maytenus aquifolium were induced to form callus and, subsequently, suspension cultures. The isolation of natural products from callus led to the identification of the cytotoxic triterpene quinonemethides, maitenin (1) and 22 beta-hydroxymaitenin (2), A rapid, sensitive and reliable reversed-phase high-performance liquid chromatography method was developed using a Cls column and isocratic elution for the determination of 1 and 2, the elaborated method gave well-resolved peaks for these compounds with good detection response and linearity in the range of 0.08-72.0 mu g. The quantification of 1 and 2 was performed by an external standard method. (C) 1998 John Wiley & Sons, Ltd.