Articulo
Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
Registro en:
issn:1615-6102
issn:0033-183X
Autor
Brentassi, María Eugenia
Franco, Mario Emilio Ernesto
Balatti, Pedro Alberto
Medina, Rocío
Bernabei, Franco
Marino de Remes Lenicov, Ana María
Institución
Resumen
In this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper <i>Dalbulus maidis</i> by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont “<i>Candidatus</i> Sulcia muelleri” (Phylum Bacteroidetes) resides in bacteriomes of <i>D. maidis</i>. Phylogenetic analysis showed that the sequence was closely allied to others found in representatives of the subfamily Deltocephalinae. We failed to amplify other sequences as “<i>Candidatus</i> Nasuia deltocephalinicola,” a co-primary symbiont frequently associated to deltocephaline leafhoppers. In addition, a metagenetic analysis carried out in order to investigate the presence of other bacteriome-associated bacteria of <i>D. maidis</i> showed that the sequence of <i>Sulcia</i> accounted for 98.56 % of all the sequences. Histological and ultrastructural observations showed that microorganisms harbored in bacteriomes (central syncytium and cytoplasm of uninucleate bacteriocytes) look like others <i>Sulcia</i> described in hemipteran species and they were transovarially transmitted from mother to offspring which is typical of obligate endosymbionts. The only presence of <i>Sulcia</i> in the bacteriomes of <i>D. maidis</i> was discussed. Facultad de Ciencias Naturales y Museo Centro de Investigaciones en Fitopatología Centro de Investigación y Desarrollo en Fermentaciones Industriales