Artículos de revistas
Development of a set of SNP markers for population genetics studies of Ipe (Handroanthus sp.), a valuable tree genus from Latin America
Fecha
2018-12-01Registro en:
Conservation Genetics Resources. Dordrecht: Springer, v. 10, n. 4, p. 779-781, 2018.
1877-7252
10.1007/s12686-017-0928-5
WOS:000456651300042
Autor
Universidade Estadual Paulista (Unesp)
Thunen Inst Forest Genet
Univ Autonoma Gabriel Rene Moreno
Univ Guyane
Inst Florestal Sao Paulo
INRA Pierroton
Institución
Resumen
A combination of restriction associated DNA sequencing (RADSeq) and low coverage MiSeq genome sequencing was used for the development of single nucleotide polymorphisms (SNP) and INDEL (insertion/deletions) genetic markers for Ipe (Handroanthus sp.). Of the 402 putative loci identified, 389 SNPs and INDELs (315 nuclear SPNs, six chloroplast INDELs, 15 chloroplast SNPs, 12 mitochondrial INDELs and 41 mitochondrial SNPs) were successfully genotyped at 93 individuals from Brazil, Bolivia and French Guiana using a - MassARRAY (R) iPLEX (TM) platform. This set of markers will be invaluable for population genetics, phylogeography and DNA fingerprinting studies.