Artigo
Cytochemical, immunocytochemical and ultrastructural observations on leukocytes and thrombocytes of fat snook (Centropomus parallelus)
Fecha
2011-10-01Registro en:
Fish & Shellfish Immunology. London: Academic Press Ltd- Elsevier B.V., v. 31, n. 4, p. 571-577, 2011.
1050-4648
10.1016/j.fsi.2011.07.019
WOS:000295020000008
Autor
Silva, Wemeson F. da [UNIFESP]
Egami, Mizue Imoto [UNIFESP]
Santos, Antenor A.
Antoniazzi, Marta M.
Silva, Marcelo [UNIFESP]
Gutierre, Robson C. [UNIFESP]
Paiva, Maria Jose R.
Institución
Resumen
The cytochemical, immunocytochemical and ultrastructural characteristics of leukocytes and thrombocytes in the peripheral blood of the fat snook (Centropomus paralellus) - a fish occurring in Brazil were investigated. the cytochemical methods were performed to demonstrate four enzymatic reactions o-toluidine-hydrogen peroxide, naphtol AS-MX phosphate, naphtol AS-BI phosphate and alpha-naphtil acetate to detect myeloperoxidase (MPO), alkaline phosphatase (ALP), acid phosphatase (ACP) and non-specific esterase (alpha-NAE), respectively and two non-enzymatic ones Periodic-Acid Schiff (PAS) and Sudan black B (SBB) to detect the occurrence of glycogen and phospholipids, respectively. Immunocytochemical method utilizing polyclonal rabbit antibody against mammal metalloproteinases (MMPs) 2 and 9 were done. Standard method for Electron Microscopy (EM) was applied for the ultrastructural study. the cytochemical reactions were positive in neutrophils for MPO, ACP, alpha-NAE, glycogen and phospholipids; in lymphocytes for ACP and alpha-NAE; in monocytes for ACP and alpha-NAE and in thrombocytes for ACP, alpha-NAE and glycogen. Only neutrophils were positive for MMPs 2 and 9, and none of the cells studied were positive for ALP. Ultrastructurally: 1) neutrophil showed a spherical shape with a spherical, indented or lobulated euchromatic nucleus, and cytoplasm containing granules of varied sizes and mitochondria of varied shapes and sizes. the nucleus/cytoplasm relation and the size of granules suggest neutrophil maturation in peripheral blood; 2) lymphocytes showed partially heterochromatic nucleus and minimal cytoplasm; 3) monocytes had long cytoplasmic projections, an indented nucleus, evident nucleolus and cytoplasm with granules of varied sizes and vacuoles; 4) thrombocytes were predominantly elliptical or roughly spherical in shape, had a partially heterochromatic nucleus and cytoplasm containing electron-dense granules, intricate canalicular system and vacuoles occasionally holding phagocytic material. (C) 2011 Elsevier B.V. All rights reserved.