Artigo
Crystallization and preliminary X-ray diffraction analysis of crotoxin B from Crotalus durissus collilineatus venom
Fecha
2009-10-01Registro en:
Acta Crystallographica Section F-structural Biology and Crystallization Communications. Malden: Wiley-blackwell Publishing, Inc, v. 65, p. 1011-1013, 2009.
1744-3091
10.1107/S1744309109032631
WOS:000270387700012
WOS000270387700012.pdf
Autor
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Resumen
Crotoxin B is a basic phospholipase A(2) found in the venom of several Crotalus durissus ssp. rattlesnakes and is one of the subunits that constitute crotoxin, the main component of the venom of these snakes. This heterodimeric toxin is related to important envenomation effects such as neurological disorders, myotoxicity and renal failure. Although crotoxin was first crystallized in 1938, the first structural data only became available in 2007 (for crotoxin B from C. durissus terrificus) and showed an ambiguous result for the biological assembly, which could be either dimeric or tetrameric. In this work, the crystallization, X-ray diffraction data collection at 2.2 angstrom resolution and molecular-replacement solution of a dimeric complex formed by two crotoxin B isoforms from C. durissus collilineatus venom is presented.