Generación de Nuevo Conocimiento: Artículos publicados en revistas especializadas - Electrónicos
Expression of Toll-like receptors 2 and 4 and its association with matrix metalloproteinases in symptomatic and asymptomatic apical periodontitis
Fecha
2019-02-26Registro en:
Fernández, A., Cárdenas, A. M., Astorga, J., Veloso, P., Alvarado, A., Merino, P., . . . Reyes-Court, D. (2019). In Hernández M. (Ed.), Expression of toll-like receptors 2 and 4 and its association with matrix metalloproteinases in symptomatic and asymptomatic apical periodontitis. Bogota: doi:10.1007/s00784-019-02861-9
Autor
Fernández, A.
Cárdenas, A. M.
Astorga, J.
Veloso, P.
Alvarado, A.
Merino, P.
Pino, D.
Reyes-Court, D.
Institución
Resumen
To determine Toll-like receptors (TLR)2 and TLR4 expression levels and associate them with matrix metalloproteinases
(MMPs) in asymptomatic apical periodontitis (AAP), symptomatic apical periodontitis (SAP), and healthy controls.
Apical tissue/lesion samples were obtained from chronic AAP (n = 35) and SAP (n = 29), and healthy periodontal
ligament (HPL, n = 10) with indication of tooth extraction, respectively. mRNA expression levels of TLR2, TLR4,
MMP-1, MMP-2, MMP-8, and MMP-13 were determined by real-time reverse-transcription polymerase chain reaction.
The data were analyzed with Kruskal-Wallis and Dunn’s pot hoc test (p < 0.05). The correlation coefficient was
obtained using the Spearman correlation (p < 0.05). TLR2, MMP-1, MMP-2, and MMP-13 mRNA levels were the
highest in SAP followed by AAP and controls (p<0.05). TLR4 and MMP-8 were over expressed in AAP and SAP
compared to HPL (p < 0.05). TLR2 positively correlated with TLR4, MMP-1, MMP-8, and MMP-13 in SAP
(p < 0.05). TLR2 and TLR4 are overexpressed in apical lesions versus healthy periodontal ligament and correlate with
collagenolytic MMPs. Particularly, TLR2 is overexpressed in SAP in association with MMP-1, MMP-8, and MMP-13.
Our results suggest that the activation of TLR2 along with MMP overexpression might contribute to SAP clinical
presentation and progression. TLRs, MMPs, and their interaction can explain the clinical presentations and evolution
of apical periodontitis and might represent key targets for new diagnostic and treatment approaches.