Artículos de revistas
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats
Fecha
1988Registro en:
KERR, I. B. et al. Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats. Revista do Instituto de Medicina Tropical, v.30, n.5, p.336-350, Sept./Oct.1988.
0036-4665
Autor
Kerr, I. B.
Araripe, J. R.
Oliveira, P. C.
Lenzi, Henrique L,
Institución
Resumen
Female albino rats were used for the sequential histopathological study of
experimental paracoccidioidomycosis. The animals were inoculated
intraperitoneally with a strain of Paracoccidioides brasiliensis in the yeast-like
phase, and sacrificed at given intervals from 1 to 168 days after inoculation; each
animal received an inoculum of 4 x 10^ cells in 0.8 ml of saline. The control group
received saline containing scrapings of the culture medium.
Tissue from the inoculation site was examined. The cellular population, the
extracellular matrix, and the presence and characteristics of fungi were analysed in
the inflammatory granulomatous process by light microscopy.
The results allowed to separate the kinetic of the inflammatory response into
three stages: 1) neutrophilic or macrophagic-neutrophilic; 2) pre-granulomatous; 3)
granulomatous.
Synthesis of the extracellular matrix began with the depositing of fibrin-like
material, and increased gradually with deposits of collagen, proteoglycans, and
glycoproteins. Parasites were present in all of the examined periods.
Recurrences of the disease were clearly shown through the concurrence of
recently-formed granulomas with older granulomas, implying that this type of
granulomatous process does not eliminate the disease, nor is it able to limit fungal
dissemination over a prolonged period of time.