Artículos de revistas
Intasome architecture and chromatin density modulate retroviral integration into nucleosome
Fecha
2015Registro en:
Retrovirology, Volumen 12, Issue 1, 2018,
17424690
10.1186/s12977-015-0145-9
Autor
Benleulmi, Salah S.
Matysiak, Julien
Henriquez, Rodrigo R.
Vaillant, Cédric
Lesbats, Paul
Calmels, Christina
Naughtin, Monica
Leon, Oscar
Skalka, Anna Marie
Ruff, Marc
Lavigne, Marc
Andreola, Marie Line
Parissi, Vincent
Institución
Resumen
© Benleulmi et al. Background: Retroviral integration depends on the interaction between intasomes, host chromatin and cellular targeting cofactors as LEDGF/p75 or BET proteins. Previous studies indicated that the retroviral integrase, by itself, may play a role in the local integration site selection within nucleosomal target DNA. We focused our study on this local association by analyzing the intrinsic properties of various retroviral intasomes to functionally accommodate different chromatin structures in the lack of other cofactors. Results: Using in vitro conditions allowing the efficient catalysis of full site integration without these cofactors, we show that distinct retroviral integrases are not equally affected by chromatin compactness. Indeed, while PFV and MLV integration reactions are favored into dense and stable nucleosomes, HIV-1 and ASV concerted integration reactions are preferred into poorly dense chromatin regions of our nucleosomal acceptor templates. Predicted nucle