Artículos de revistas
Effectiveness of batimastat, a synthetic inhibitor of matrix metalloproteinases, in neutralizing local tissue damage induced by BaP1, a hemorrhagic metalloproteinase from the venom of the snake Bothrops asper
Escalante Muñoz, Teresa
Rucavado Romero, Alexandra
Gutiérrez, José María
Batimastat (BB-94), a synthetic hydroxamate peptidomimetic matrix metalloproteinase inhibitor, was tested for its ability to inhibit proteolytic and toxic effects induced by BaP1, a 24-kDa hemorrhagic metalloproteinase isolated from the venom of Bothrops asper, the medically most important snake species in Central America and southern Mexico. Batimastat inhibited proteolytic activity on biotinylated casein, with anic50 of 80 nM. In addition, batimastat was effective in inhibiting hemorrhagic, dermonecrotic, and edema-forming activities of this metalloproteinase if incubated with the enzyme prior to the assays. When the inhibitor was administered i.m. at the site of the toxin injection without preincubation, rapidly after metalloproteinase administration, it totally abrogated the hemorrhagic and dermonecrotic effects of BaP1. Inhibition was less effective as the time lapse between toxin and batimastat injection increased, due to the extremely rapid development of BaP1-induced local tissue damage in this experimental model. On the other hand, batimastat was ineffective if administered by the i.p. route immediately after toxin injection. It is concluded that batimastat, and probably other synthetic metalloproteinase inhibitors, may become useful therapeutic tools aimed at the in situ inhibition of venom metalloproteinases, when injected at the site of the bite rapidly after envenomation.
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Tissue inhibitor of metalloproteinase-2 (TIMP-2) location in the ventral, lateral, dorsal and anterior lobes of rat prostate by immunohistochemistry Delella, Flávia K.; Justulin Jr., Luis A.; Felisbino, Sérgio L.
Tissue inhibitor of metalloproteinase-2 (TIMP-2) location in the ventral, lateral, dorsal and anterior lobes of rat prostate by immunohistochemistry Universidade Estadual de Campinas (UNICAMP); Universidade Estadual Paulista (UNESP) (2007-03-01)Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) play a major role in extracellular matrix component degradation in several normal and abnormal tissue situations; they are also found in human seminal ...
Jucá, Mário; Nunes, Benicio Luiz Bulhões Barros Paula; Menezes, Hunaldo Lima De; Gomes, Edmundo Guilherme De Almeida; Matos, Delcio (Cidade Editora Científica Ltda, 2008-09-01)The metalloproteinase-1 (MMP-1) and metalloproteinase-7 (MMP-7) are proteinases of the extracellular matrix (MEC), zinc-dependent, involved in the initial process of carcinogenesis, allowing the invasion by the tumor cell ...