Artículos de revistas
Aging impairs the ability of conventional dendritic cells to cross-prime CD8+ T cells upon stimulation with a TLR7 ligand
Fecha
2015-10Registro en:
Zacca, Estefanía; Crespo, Maria Ines; Acland Strack, Rachel Paola; Roselli, Emiliano; Núñez, Nicolás; et al.; Aging impairs the ability of conventional dendritic cells to cross-prime CD8+ T cells upon stimulation with a TLR7 ligand; Public Library of Science; Plos One; 10; 10; 10-2015
1932-6203
CONICET Digital
CONICET
Autor
Zacca, Estefanía
Crespo, Maria Ines
Acland Strack, Rachel Paola
Roselli, Emiliano
Núñez, Nicolás
Maccioni, Mariana
Maletto, Belkys Angélica
Pistoresi, Maria Cristina
Moron, Victor Gabriel
Resumen
The aging process is accompanied by altered immune system functioning and an increased risk of infection. Dendritic cells (DCs) are antigen-presenting cells that play a key role in both adaptive and innate immunity, but how aging affects DCs and their influence on immunity has not been thoroughly established. Here we examined the function of conventional DCs (cDCs) in old mice after TLR7 stimulation, focusing on their ability to cross-prime CD8+ T cells. Using polyU, a synthetic ssRNA analog, as TLR7 ligand and OVA as an antigen (Ag) model, we found that cDCs from old mice have a poor ability to stimulate a CD8+ T cell-mediated cytotoxic response. cDCs from old mice exhibit alterations in Ag-processing machinery and TLR7 activation. Remarkably, CD8α+ cDCs from old mice have an impaired ability to activate naïve CD8+ T cells and, moreover, a lower capacity to mature and to process exogenous Ag. Taken together, our results suggest that immunosenescence impacts cDC function, affecting the activation of naïve CD8+ T cells and the generation of effector cytotoxic T cells.