Artículos de revistas
Coimmunoprecipitation on Drosophila cells in culture
Fecha
2007-12Registro en:
Coimmunoprecipitation on Drosophila cells in culture; Humana Press; Methods In Molecular Biology (clifton, N.j.); 362; 12-2007; 423-427
1064-3745
1940-6029
CONICET Digital
CONICET
Autor
Ceriani, Maria Fernanda
Resumen
Coimmunoprecipitation (coIP) provides evidence that two or more proteins can be found in the same complex. It can be performed in vitro (employing in vitro transcribed and translated proteins, or proteins expressed in Escherichia coli) or from transfected cells, which assess whether the interaction takes place in a more functional context. This chapter includes a general description and guidelines to carry out coIP in transfected Schneider's cells