Artículos de revistas
Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis
Fecha
2008-12-01Registro en:
De Bruyne, Katrien; Franz, Charles M. A. P.; Vancanneyt, Marc; Schillinger, Ulrich; Mozzi, Fernanda Beatriz; et al.; Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 58; 12; 1-12-2008; 2909-2916
1466-5026
1466-5034
CONICET Digital
CONICET
Autor
De Bruyne, Katrien
Franz, Charles M. A. P.
Vancanneyt, Marc
Schillinger, Ulrich
Mozzi, Fernanda Beatriz
Font, Graciela Maria
De Vuyst, Luc
Vandamme, Peter
Resumen
A Gram-positive, small coccus-shaped lactic acid bacterium, strain LMG 23999T, was isolated from Argentinean wheat flour. 16S rRNA gene sequence analysis revealed that the phylogenetic position of the novel strain was within the genus Pediococcus, with Pediococcus stilesii, Pediococcus pentosaceus and Pediococcus acidilactici as its closest relatives (97.7, 97.3 and 96.9% gene sequence similarity, respectively). Fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and whole-cell protein electrophoresis confirmed the unique taxonomic status of the novel strain. DNA-DNA hybridizations, DNA G+C content determination, comparative sequence analysis of the pheS, rpoA and atpA genes and physiological and biochemical characterization demonstrated that strain LMG 23999T (=CCUG 54535T=CRL 776T) represents a novel species for which the name Pediococcus argentinicus sp. nov. is proposed. Multi-locus sequence analysis based on pheS, rpoA and atpA genes was found to be a suitable method for the identification of species of the genus Pediococcus.