Artículos de revistas
Design of stable metabolic networks
Fecha
2017-08Registro en:
Di Maggio, Jimena Andrea; Blanco, Anibal Manuel; Bandoni, Jose Alberto; Diaz Ricci, Juan Carlos; Díaz, María Soledad; Design of stable metabolic networks; Wiley VCH Verlag; Engineering In Life Sciences; 17; 8; 8-2017; 908-915
1618-0240
CONICET Digital
CONICET
Autor
Di Maggio, Jimena Andrea
Blanco, Anibal Manuel
Bandoni, Jose Alberto
Diaz Ricci, Juan Carlos
Díaz, María Soledad
Resumen
In this work, we propose eigenvalue optimization combined with Lyapunov theory concepts to ensure stability of the Embden—Meyerhof–Parnas pathway, the pentosephosphate pathway, the phosphotransferase system and fermentation reactions of Escherichia coli. We address the design of a metabolic network for the maximization of different metabolite production rates. The first case study focuses on serine production, based on a model that consists of 18 differential equations corresponding to dynamic mass balances for extracellular glucose and intracellular metabolites, and thirty kinetic rate expressions. A second case study addresses the design problem to maximize ethanol production, based on a dynamic model that involves mass balancesfor 25 metabolites and 38 kinetic rate equations. The nonlinear optimization problem including stability constraints has been solved with reduced space Successive Quadratic Programming techniques. Numerical results provide useful insights on the stability properties of the studied kinetic models.