Artículos de revistas
Paracellular absorption in laboratory mice: Molecule size-dependent but low capacity
Fasulo, Verónica; Zhang, Zhiqiang; Price, Edwin; Chediack, Juan Gabriel; Karasov, William H.; et al.; Paracellular absorption in laboratory mice: Molecule size-dependent but low capacity; Elsevier; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 164; 1; 2-2013; 71-76
Chediack, Juan Gabriel
Karasov, William H.
Caviedes Vidal, Enrique Juan Raul
Water-soluble nutrients are absorbed by the small intestine via transcellular and paracellular processes. The capacity for paracellular absorption seems lower in nonfliers than in fliers, although that conclusion rests largely on a comparison of relatively larger nonflying mammals (> 155 g) and relatively smaller flying birds (< 155 g). We report on paracellular absorption in laboratory mice, the smallest nonflying mammal species studied to date. Using a standard pharmacokinetic technique, we measured the extent of absorption (fractional absorption = f) of inert carbohydrate probes: l-arabinose (Mr = 150.13 Da) and cellobiose (342.3) that are absorbed exclusively by the paracellular route, and 3-O-methyl d-glucose (3OMD-glucose) (Mr = 194) absorbed both paracellularly and transcellularly. f was measured accurately in urine collection trials of 5?10 h duration. Absorption of 3OMD-glucose by mice was essentially complete (f = 0.95 ± 0.07) and much higher than that for l-arabinose (f = 0.21 ± 0.02), indicating that in mice, like other nonflying mammals, > 80% of glucose is absorbed by mediated process(es) rather than the passive, paracellular route. As in all other vertebrates, absorption of cellobiose (f = 0.13 ± 0.02) was even lower than that for l-arabinose, suggesting an equivalent molecular size cut-off for flying and nonflying animals and thus a comparable effective TJ aperture. An important ecological implication is that smaller water-soluble plant secondary metabolites that have been shown to be absorbed by the paracellular path in cell culture, such as phenolics and alkaloids, might be absorbed in substantial amounts by bats and small birds relative to nonflying mammals such as mice.