Artículos de revistas
Palmitoleic acid (n-7) increases white adipocyte lipolysis and lipase content in a PPARα-dependent manner
Fecha
2013-11-01Registro en:
AJP - Endocrinology and Metabolism, Bethesda, v.305, n.9, p.e1093-e1102, 2013
10.1152/ajpendo.00082.2013
Autor
Lopes, Andressa Bolsoni
Festuccia, William Tadeu Lara
Farias, Talita da Silva Mendes de
Chimin, Patricia
Leal, Francisco Leonardo Tôrres
Derogis, Priscilla Bento Matos Cruz
Andrade, Paula Bresciani Martins de
Miyamoto, Sayuri
Lima, Fabio Bessa
Curi, Rui
Alonso-Vale, Maria Isabel C.
Institución
Resumen
We investigated whether palmitoleic acid, a fatty acid that enhances whole body glucose disposal and suppresses hepatic steatosis, modulates triacylglycerol (TAG) metabolism in adipocytes. For this, both differentiated 3T3-L1 cells treated with either palmitoleic acid (16:1n7, 200 μM) or palmitic acid (16:0, 200 μM) for 24 h and primary adipocytes from wild-type or PPARα-deficient mice treated with 16:1n7 (300 mg•kg(-1)•day(-1)) or oleic acid (18:1n9, 300 mg•kg(-1)•day(-1)) by gavage for 10 days were evaluated for lipolysis, TAG, and glycerol 3-phosphate synthesis and gene and protein expression profile. Treatment of differentiated 3T3-L1 cells with 16:1n7, but not 16:0, increased basal and isoproterenol-stimulated lipolysis, mRNA levels of adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) and protein content of ATGL and pSer(660)-HSL. Such increase in lipolysis induced by 16:1n7, which can be prevented by pharmacological inhibition of PPARα, was associated with higher rates of PPARα binding to DNA. In contrast to lipolysis, both 16:1n7 and 16:0 increased fatty acid incorporation into TAG and glycerol 3-phosphate synthesis from glucose without affecting glyceroneogenesis and glycerokinase expression. Corroborating in vitro findings, treatment of wild-type but not PPARα-deficient mice with 16:1n7 increased primary adipocyte basal and stimulated lipolysis and ATGL and HSL mRNA levels. In contrast to lipolysis, however, 16:1n7 treatment increased fatty acid incorporation into TAG and glycerol 3-phosphate synthesis from glucose in both wild-type and PPARα-deficient mice. In conclusion, palmitoleic acid increases adipocyte lipolysis and lipases by a mechanism that requires a functional PPARα