Artículos de revistas
Basic aminopeptidase activity is an emerging biomarker in collagen-induced rheumatoid arthritis
Fecha
2011Registro en:
REGULATORY PEPTIDES, v.167, n.2/Mar, p.215-221, 2011
0167-0115
10.1016/j.regpep.2011.02.012
Autor
MENDES, Mariana Trivilin
MURARI-DO-NASCIMENTO, Stephanie
TORRIGO, Isis Rossetti
ALPONTI, Rafaela Fadoni
YAMASAKI, Simone Cristina
SILVEIRA, Paulo Flavio
Institución
Resumen
The objective of this study was to investigate the catalytic activity of basic aminopeptidase (APB) and its association with periarticular edema and circulating tumor necrosis factor (TNF)-alpha and type II collagen (CII) antibodies (AACII) in a rat model of rheumatoid arthritis (RA) induced by CII (CIA). Edema does not occur in part of CH-treated, even when AACII is higher than in control. TNF-alpha is detectable only in edematous CII-treated. APB in synovial membrane is predominantly a membrane-bound activity also present in soluble form and with higher activity in edematous than in non-edematous CH-treated or control. Synovial fluid and blood plasma have lower APB in non-edematous than in edematous CII-treated or control. In peripheral blood mononuclear cells (PBMCs) the highest levels of APB are found in soluble form in control and in membrane-bound form in non-edematous CII-treated. CII treatment distinguishes two categories of rats: one with arthritic edema, high AACII, detectable TNF-alpha, high soluble and membrane-bound APB in synovial membrane and low APB in the soluble fraction of PBMCs, and another without edema and with high AACII, undetectable TNF-alpha, low APB in the synovial fluid and blood plasma and high APB in the membrane-bound fraction of PBMCs. Data suggest that APB and CIA are strongly related. (C) 2011 Elsevier B.V. All rights reserved.