Artículos de revistas
Creatine Supplementation Prevents the Accumulation of Fat in the Livers of Rats Fed a High-Fat Diet
Fecha
2011Registro en:
JOURNAL OF NUTRITION, v.141, n.10, p.1799-1804, 2011
0022-3166
10.3945/jn.111.144857
Autor
DEMINICE, Rafael
SILVA, Robin P. da
LAMARRE, Simon G.
BROWN, Colin
FUREY, George N.
MCCARTER, Shannon A.
JORDAO, Alceu Afonso
KELLY, Karen B.
KING-JONES, Kirst
JACOBS, Rene L.
BROSNAN, Margaret E.
BROSNAN, John T.
Institución
Resumen
The aim of the present study was to examine the effects of creatine supplementation on liver fat accumulation induced by a high-fat diet in rats. Rats were fed 1 of 3 different diets for 3 wk: a control liquid diet (C), a high-fat liquid diet (HF), or a high-fat liquid diet supplemented with creatine (HFC). The C and HF diets contained, respectively, 35 and 71% of energy derived from fat. Creatine supplementation involved the addition of 1% (wt:v) of creatine monohydrate to the liquid diet. The HF diet increased total liver fat concentration, liver TG, and liver TBARS and decreased the hepatic S-adenosylmethionine (SAM) concentration. Creatine supplementation normalized all of these perturbations. Creatine supplementation significantly decreased the renal activity of L-arginine:glycine amidinotransferase and plasma guanidinoacetate and prevented the decrease in hepatic SAM concentration in rats fed the HF diet. However, there was no change in either the phosphatidylcholine:phosphatidylethanolamine (PE) ratio or PE N-methyltransferase activity. The HF diet decreased mRNA for PPAR as well as 2 of its targets, carnitine palmitoyltransferase and long-chain acylCoA dehydrogenase. Creatine supplementation normalized these mRNA levels. In conclusion, creatine supplementation prevented the fatty liver induced by feeding rats a HF diet, probably by normalization of the expression of key genes of beta-oxidation. J. Nutr. 141: 1799-1804, 2011.