Artículos de revistas
The isolation of antioxidant enzymes from mature tomato (cv. Micro-Tom) plants
Fecha
2008Registro en:
HORTSCIENCE, v.43, n.5, p.1608-1610, 2008
0018-5345
Autor
GRATAO, Priscila L.
MONTEIRO, Carolina C.
PERES, Lazaro E. P.
AZEVEDO, Ricardo Antunes
Institución
Resumen
The activity of catalase (CAT), guaiacol peroxidase (GPOX), ascorbate peroxidase (APX), glutathione reductase (GR), and the isoenzymes of superoxide dismutase (SOD) were determined in the organs of tomato (Lycopersicon esculentum) cultivar Micro-Tom after 104 days of development. The total activities of CAT, GPOX, and GR were higher in the stem than in others tissues, whereas the stem exhibited the lowest APX activity. Activity staining analysis following gel electrophoresis revealed the existence of four SOD isoenzymes in leaves, three in fruits, but only two in the roots and stems. This characterization is essential for an investigation into the effect of abiotic and biotic stresses on the oxidative stress responses by this plant model system.