Artículos de revistas
A study of cell disruption of Candida mogii by glass bead mill for the recovery of xylose reductase
Fecha
2008Registro en:
SEPARATION AND PURIFICATION TECHNOLOGY, v.63, n.3, p.706-709, 2008
1383-5866
10.1016/j.seppur.2008.06.019
Autor
MAYERHOFF, Zea D. V. L.
FRANCO, Telma T.
ROBERTO, Ines C.
Institución
Resumen
The release of xylose reductase (XR) from Candida mogii by cell disruption in a glass beads mill was studied using an experimental design. Statistical analysis of the results indicated that XR volumetric activity increases by using lower glass beads diameter and cell concentration, and by increasing the number of agitation pulses. Based on results attained in experimental design, assays were carried out aiming at the maximization of XR release. Under optimized conditions (300 mu m glass beads, 45 g/l of cell concentration and 50 pulses), the XR volumetric activity reach 0.683 U/ml. Disruption with glass beads showed to be the most efficient method for XR release when compared to sonication process. The highest specific activity (0.175 U/mg of protein) was found in extracts obtained by suspension freezing and thawing, which suggests that this method can be used as a selective process of cell disruption for XR release. (c) 2008 Elsevier B.V. All rights reserved.