Artículos de revistas
Quality Of Sweat Test (st) Based On The Proportion Of Sweat Sodium (na) And Sweat Chloride (cl) As Diagnostic Parameter Of Cystic Fibrosis: Are We On The Right Way?
Registro en:
Diagnostic Pathology. Biomed Central Ltd, v. 11, p. , 2016.
1746-1596
WOS:000386179900001
10.1186/s13000-016-0555-6
Autor
Faria
Alethea Guimaraes; Lima Marson
Fernando Augusto; de Souza Gomez
Carla Cristina; Goncalves de Oliveira Ribeiro
Maria Angela; Morais
Lucas Brioschi; Servidoni
Maria de Fatima; Bertuzzo
Carmen Silvia; Sakano
Eulalia; Goto
Maura; Paschoal
Ilma Aparecida; Pereira
Monica Corso; Hessel
Gabriel; Levy
Carlos Emilio; Dalbo Contrera Toro
Adyleia Aparecida; Peixoto
Andressa Oliveira; Ribeiro Simoes
Maria Cristina; Lomazi
Elizete Aparecida; Negrao Nogueira
Roberto Jose; Ribeiro
Antonio Fernando; Ribeiro
Jose Dirceu
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) To assess the quality of sweat test (ST) based on the proportion of sweat sodium and sweat chloride as diagnostic parameter of cystic fibrosis (CF). Methods: A retrospective study of 5,721 sweat samples and subsequent descriptive analysis were carried out. The test was considered "of good quality" (correct) when: (i) sweat chloride was lower than 60 mEq/L, and sweat sodium was higher than sweat chloride; (ii) sweat chloride was higher than 60 mEq/L, and sweat sodium was lower than sweat chloride. Results: The study included 5,692/5,721 sweat samples of ST which had been requested due to clinical presentations compatible with CF and/or neonatal screenings with altered immunoreactive trypsinogen values. Considering the proportion of sweat sodium and sweat chloride as ST quality parameter, the test was performed correctly in 5,023/5,692 (88.2 %) sweat samples. The sweat chloride test results were grouped into four reference ranges for chloride (i) chloride >= 30 mEq/L: 3,651/5,692 (64.1 %); (ii) chloride < 30 mEq/L to < 40 mEq/L: 652/5,692 (11.5 %); (iii) >= 40 mEq/L to < 60 mEq/L: 673/5,692 (11.8 %); (iv) >= 60 mEq/L: 716/5,692 (12.6 %). In the comparative analysis, there was no association between ST quality and: (i) symptoms to indicate a ST [respiratory (p = 0.084), digestive (p = 0.753), nutritional (p = 0.824), and others (p = 0.136)], (ii) sweat weight (p = 0.416). However, there was a positive association with: (i) gender, (ii) results of ST (p < 0.001), (iii) chloride/sodium ratio (p < 0.001), (iv) subject's age at the time of ST [grouped according to category (p < 0.001) and numerical order (p < 0.001)]. For the subset of 169 patients with CF and two CFTR mutations Class I, II and/or III, in comparative analysis, there was a positive association with: (i) sweat chloride/sodium ratio (p < 0.001), (ii) sweat chloride values (p = 0.047), (iii) subject's age at the time of the ST grouped by numerical order (p = 0.001). Conclusions: Considering that the quality of ST can be assessed by levels of sweat sodium and sweat chloride, an increasing number of low-quality tests could be observed in our sweat samples. The quality of the test was associated with important factors, such as gender, CF diagnosis, and subjects' age. 11 103 FALM: Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/12939-4, 2015/12858-5] Fundo de Apoio a Pesquisa ao Ensino e a Extensao da Universidade Estadual de Campinas [0648/2015] JDR: FAPESP [2011/18845-1] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)