Artículos de revistas
Assisted-selection Of Naturally Caffeine-free Coffee Cultivars-characterization Of Snps From A Methyltransferase Gene
Registro en:
Molecular Breeding. Springer, v. 37, p. , 2017.
1380-3743
1572-9788
WOS:000400080100008
10.1007/s11032-017-0636-6
Autor
Favoretto
Patricia; da Silva
Carla Cristina; Tavares
Aline Gomes; Giatti
Gabriela; Moraes
Patricia Favoretto; Vargas Lobato
Mary Tulia; Silvarolla
Maria Bernadete; Oliveiro-Filho
Guerreiro; Maluf
Mirian Perez
Institución
Resumen
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Breeding of caffeine-free coffee cultivars require tools for an early selection of progenies bearing this later trait. Genes from caffeine synthesis and degradation represent major targets for the development of molecular markers for assisted selection. In this study, we characterized SNPs identified on the caffeine synthase gene from AC1 mutant, a naturally caffeine-free arabica coffee plant. Molecular analysis of normal and mutant sequences indicates the occurrence of SNPs in protein domains, potentially associated with caffeine synthesis in coffee. Progenies F-2, F1BC1 and BC from crosses of AC mutants and elite cultivars were evaluated regarding caffeine content in grains and genomic segregation profile of selected SNPs. Genotyping analysis allowed the discrimination between homozygous and heterozygous plants. Quantification of caffeine content indicated a significant variability among progenies and a low frequency of caffeine-free plants. Statistical analyses of genotyping and phenotyping results showed significant association between presence of selected SNPs and reduced caffeine content. Moreover, this association occurs through all evaluated genetic backgrounds and generations, indicating an inheritance stability of both trait and markers. The molecular markers described here represent a successful case of assisted-selection in coffee, indicating their potential use for breeding of caffeine-free cultivars. 37 3 Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES) Brazilian Consortium for Coffee Research and Development (CBPD Cafe) Studies and Projects Financing Agency (Finep) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)