Artículos de revistas
Direct Visualization Of Neurotransmitters In Rat Brain Slices By Desorption Electrospray Ionization Mass Spectrometry Imaging (desi - Ms)
Registro en:
Journal Of The American Society For Mass Spectrometry. Springer, v. 27, p. 1944 - 1951, 2016.
1044-0305
1879-1123
WOS:000387335700008
10.1007/s13361-016-1475-0
Autor
Fernandes
Anna Maria A. P.; Vendramini
Pedro H.; Galaverna
Renan; Schwab
Nicolas V.; Alberici
Luciane C.; Augusti
Rodinei; Castilho
Roger F.; Eberlin
Marcos N.
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Mass spectrometry imaging (MSI) of neurotransmitters has so far been mainly performed by matrix-assisted laser desorption/ionization (MALDI) where derivatization reagents, deuterated matrix and/or high resolution, or tandem MS have been applied to circumvent problems with interfering ion peaks from matrix and from isobaric species. We herein describe the application of desorption electrospray ionization mass spectrometry imaging (DESI)-MSI in rat brain coronal and sagittal slices for direct spatial monitoring of neurotransmitters and choline with no need of derivatization reagents and/or deuterated materials. The amino acids gamma-aminobutyric (GABA), glutamate, aspartate, serine, as well as acetylcholine, dopamine, and choline were successfully imaged using a commercial DESI source coupled to a hybrid quadrupole-Orbitrap mass spectrometer. The spatial distribution of the analyzed compounds in different brain regions was determined. We conclude that the ambient matrix-free DESI-MSI is suitable for neurotransmitter imaging and could be applied in studies that involve evaluation of imbalances in neurotransmitters levels. 27 12 1944 1951 Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [11/50400-0, 10/51677-2] Conselho Nacional de Desenvolvimento Cientifico e Tecnologico CNPq [150781/2014-8] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)