Artículos de revistas
Application Of A Nanostructured Platform And Imprinted Sol-gel Film For Determination Of Chlorogenic Acid In Food Samples
Registro en:
1873-3573
Talanta. ELSEVIER SCIENCE BV, n. 156, p. 119 - 125.
1873-3573
WOS:000378182900017
10.1016/j.talanta.2016.05.020
Autor
Ribeiro
CM; Miguel
EM; Silva
JD; da Silva
CB; Goulart
MOF; Kubota
LT; Gonzaga
FB; Santos
WJR; Lima
PR
Institución
Resumen
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Chlorogenic acid (CGA) is a polyphenol derivative that widely exists in higher plants like fruits, vegetables, black teas, and some traditional Chinese medicines. In this work, we have proposed a sensitive and selective electrochemical sensor for detection of CGA. The sensor was based on a glassy carbon electrode (GCE) modified with a functional platform by grafting vinyltrimethoxysilane (VTMS) in multi walled carbon nanotubes (MWCNTs) and covered by a molecularly imprinted siloxane (MIS) film prepared using the sol-gel process. The VTMS was grafted onto the surface of the MWCNTs via in situ free radical polymerization. The MIS was obtained from the acid-catalyzed hydrolysis/condensation of a solution consisting of tetraethoxysilane (TEOS), phenyltriethoxysilane (PTEOS), (3-aminopropyl)tri-methoxysilane (APTMS), and CGA as a template molecule. The modification procedure was evaluated by differential pulse voltammetry (DPV) and scanning electron microscopy (SEM). Under optimized operational conditions, a linear response was obtained covering a concentration ranging from 0.08 mu mol L-1 to 500 mu mol L-1 with a detection limit (LOD) of 0.032 mu mol L-1. The proposed sensor was applied to CGA determination in coffee, tomato, and apple samples with recoveries ranging from 99.3% to 108.6%, showing a promising potential application in food samples. Additionally, the imprinted sensor showed a significantly higher affinity for target CGA than the non-imprinted siloxane (NIS) sensor. (C) 2016 Elsevier B.V. All rights reserved. 156
119 125 Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [550107/2012-6] FAPEAL [02/2011] IFAL [02/2016, 02/2013, 02/2014] Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)