Artículos de revistas
Insulin Receptor Has Tyrosine Kinase Activity Toward Shc In Rat Liver.
Registro en:
Brazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 31, n. 11, p. 1415-9, 1998-Nov.
0100-879X
9921277
Autor
Páez-Espinosa, E V
Carvalho, C R
Velloso, L A
Saad, M J
Institución
Resumen
Insulin induces tyrosine phosphorylation of Shc in cell cultures and in insulin-sensitive tissues of the intact rat. However, the ability of insulin receptor (IR) tyrosine kinase to phosphorylate Shc has not been previously demonstrated. In the present study, we investigated insulin-induced IR tyrosine kinase activity towards Shc. Insulin receptor was immunoprecipitated from liver extracts, before and after a very low dose of insulin into the portal vein, and incubated with immunopurified Shc from liver of untreated rats. The kinase assay was performed in vitro in the presence of exogenous ATP and the phosphorylation level was quantified by immunoblotting with antiphosphotyrosine antibody. The results demonstrate that Shc interacted with insulin receptor after infusion of insulin, and, more important, there was insulin receptor kinase activity towards immunopurified Shc. The description of this pathway in animal tissue may have an important role in insulin receptor tyrosine kinase activity toward mitogenic transduction pathways. 31 1415-9
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Efeito do consumo das proteínas, peptídeos e aminoácidos do soro do leite nas heat shock proteins (HSPs) e parâmetros relacionados em ratos = Effect of the intake of whey proteins, their peptides and amino acids on the heat shock proteins (HSPs) and health related parameters in rats
Moura, Carolina Soares de, 1988- -
ProNA2020 predicts protein-DNA, protein-RNA, and protein-protein binding proteins and residues from sequence
Qiu, J.; Bernhofer, M.; Heinzinger, M.; Kemper, S.; Norambuena Arenas, Tomás; Melo Ledermann, Francisco Javier; Rost, B. (2020)