Artículos de revistas
Polyester-toner electrophoresis microchips with improved analytical performance and extended lifetime
Registro en:
Electrophoresis. Wiley-blackwell, v. 33, n. 17, n. 2660, n. 2667, 2012.
0173-0835
WOS:000308581100006
10.1002/elps.201200009
Autor
Gabriel, EFM
Duarte, GF
Garcia, PD
de Jesus, DP
Coltro, WKT
Institución
Resumen
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) This paper reports the fabrication of polyester-toner (PT) electrophoresis microchips with improved analytical performance and extended lifetime. This has been achieved with a better understanding about the EOF generation and the influence of some parameters including the channel dimensions (width and depth), the injection mode, and the addition of organic solvent to the running buffer. The analytical performance of the PT devices was investigated using a capacitively coupled contactless conductivity detector and inorganic cations as model analytes. The proposed devices have exhibited EOF values of (3.4 +/- 0.2) x 10-4 cm2 V-1 s-1 with good stability over 25 consecutive runs. It has been found that the EOF magnitude depends on the channel dimension, i.e. the wider the channel, the higher the EOF value. The separation efficiency for inorganic cations ranged from 13 000 to 50 000 plates/m. The LOD found for K+, Na+, and Li+ were 4.2, 7.3, and 23 mu M, respectively. In addition, the same PT device has been used by three consecutive days. Lately, due to improved analytical performance, it was carried out by the first time the detection of inorganic cations in real samples such as energetic drinks and pharmaceutical formulations. 33 17 SI 2660 2667 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) CNPq [477067/2010-7]