Artículos de revistas
Mitochondrial oxidative stress after global brain ischemia in rats
Neuroscience Letters. Elsevier Ireland Ltd, v. 334, n. 2, n. 111, n. 114, 2002.
Vulnerable neurons in the hippocampus die 2-3 days after transient global brain ischemia. In the present study, rat brain mitochondria were isolated at different time points (4 h, 24 h and 48 h) after transient global ischemia. Detection of mitochondrially-generated reactive oxygen species, measured through dichlorodihydrofluorescein oxidation, was increased up to 40% relative to control in hippocampal mitochondria at 4 h and 48 h of reperfusion. Ischemia-stimulated oxidative stress was observed with mitochondria oxidizing substrates linked to nicotinamide adenine dinucleotide or flavin adenine dinucleotide, but not in the presence of the respiratory chain inhibitor antimycin A. A slightly decreased Ca2+ uptake capacity was observed in hippocampal mitochondria during reperfusion. We conclude that transient brain ischemia induces oxidative stress in hippocampal mitochondria. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.3342111114