Actas de congresos
Sugarcane Bagasse Pulps: Biobleaching With Commercial Cartazyme Hs And With Bacillus Pumilus Xylanase
Registro en:
Applied Biochemistry And Biotechnology - Part A Enzyme Engineering And Biotechnology. , v. 121, n. 1-3, p. 171 - 181, 2005.
2732289
2-s2.0-18844418981
Autor
Moriya R.Y.
Goncalves A.R.
Duarte M.C.T.
Institución
Resumen
Organosolv (ethanol/water and acetosolv) pulps were treated with Bacillus pumilus xylanase for 4, 8, and 12 h and compared with commercial Cartazyme HS xylanase-treated pulps. Treatment of ethanol/water pulps with B. pumilus xylanase increased viscosity by 40% in 8 h of treatment compared with pulps treated without enzyme. However, acetosolv pulps treated with B. pumilus xylanase lost viscosity. Ethanol/water pulps treated with Cartazyme had a viscosity of 18.5 cP in 4 h of treatment. In the acetosolv pulps treated with commercial enzyme, the loss of viscosity was 20% compared with pulps treated without enzyme. Ethanol/water pulps treated with B. pumilus and Cartazyme had similar effects: a 44% reduction in kappa number for pulps treated with enzyme followed by alkaline extraction compared with pulps treated with alkaline extraction. In acetosolv pulps treated with B. pumilus, the kappa number was from 12 to 18, compared with pulps treated without enzyme, which had a 40% reduction in 4 and 12 h and a 60% reduction in 8 h. Cartazyme-treated acetosolv pulps had a kappa number of 14 in 4 and 8 h of treatment. For 12 h of treatment, the kappa number was 8. Fourier transform infrared spectra of the pulps showed that enzyme-treated pulps had changes in the 1000 cm-1 absorption owing to a C-O bond present in esters. Using principal component analysis, it is possible to differentiate the unbleached pulps and enzyme-treated pulps. Copyright © 2005 by Humana Press Inc. All rights of any nature whatsoever reserved. 121 1-3 171 181 Bajpai, P., (1999) Biotechnol. Prog., 15 (2), pp. 147-157 Senior, D.J., Mayers, P.R., Saddler, J.N., (1991) Biotechnol. Bioeng., 37, pp. 274-279 Viikari, L., Kantelinen, A., Buchert, J., Puls, J., (1994) Appl. Microbiol. Biotechnol., 41, pp. 124-129 Kulkarni, N., Rao, M., (1996) J. Biotechnol., 51, pp. 167-173 Sholam, Y., Schwartz, Z., Khasin, A., Gat, O., Zosim, Z., Rosemberg, E., (1992) Biodegradation, 3, pp. 207-218 Bim, M.A., Franco, T.T., (2000) J. Chromatogr., 743, pp. 349-356 Dhillon, A., Gupta, J.K., Jauhari, B.M., Khanna, S., (1999) Bioresour. Technol., 73, pp. 273-277 Bajpai, P., Bhardwaj, N.K., Bajpai, P.K., Jauhari, M.B., (1994) J. Biotechnol., 38, pp. 1-6 Hinman, R.L., (1994) Chemtech, 24 (6), pp. 45-48 Wizard, N., Esterbauer, H., Steiner, W., Gomes, J., (1990), A patent 1030/90Duarte, M.C.T., Portugal, E.P., Ponezi, A.N., Bim, M.A., Tagliari, C.V., (1999) Bioresour. Technol., 68, pp. 49-53 Deshpande, V., Hinge, J., Rao, M., (1990) Biochem. Biophys. Acta, 1041, pp. 172-177 Benar, P., (1992), Ms thesis, UNIC AMP/Institute de Química, Campinas-SP, BrazilGonçalves, A.R., Ruzene, S., (2003) Appl. Biochem. Biotechnol., pp. 105-108 Bailey, M.J., Biely, P., Pourtanen, K., (1992) J. Biotechnol., 23, pp. 257-270 (1985) TAPPI Standard Methods, , T. 236 cm-85 (1992) TAPPI Standard Methods, , T. 230 om-82 Rocha, G.J.M., (2000), PhD thesis, São Carlos/Universidade de São Paulo, BrazilFaix, O., Böttcher, J.H., Berlelt, E., Using FTIR spectroscopy and FTIR microscopy for the examination of wood and wood tissue (1992) 8th International Conference in Fourier Transform Spectroscopy SPIE 1575, pp. 428-430. , Heise, M., Korte, E. H., and Siesler, H. W., eds., The International Society for Optical Engineering, San Diego, CA Morohoshi, N., (1991) Wood and Cellulosic Chemistry, pp. 331-392. , Hon, D. N. S. and Shiraishi, N., eds., Marcel Dekker, New York Scarminio, I.S., Bruns, R.E., (1989) Trends Anal. Chem., 8, pp. 326-327 Viikari, L., Tenkanen, M., Ratto, M., Bucert, J., Kantelinem, A., Bailey, M., Sundquist, J., Linko, M., (1992) Biotechnology in Pulp and Paper Industry, pp. 101-106. , Kuwahara, M. and Shimada, M. (eds.), UNI Publishers, Toyko, Japan Da Silva, R., Yim, D.K., Park, Y.K., (1994) J. Ferment. Bioeng., 77, pp. 109-111