Artículos de revistas
Extraction, Partial Characterization And Susceptibility To Hg2+ Of Acid Phosphatase From The Microalgae Pseudokirchneriella Subcapitata [extração, Caracterização Parcial E Susceptibilidade Ao Hg2+ Da Fosfatase ácida Da Microalga Pseudokirchneriella Subcapitata]
Registro en:
Scientia Agricola. , v. 66, n. 5, p. 634 - 642, 2009.
1039016
2-s2.0-70350391849
Autor
Jonsson C.M.
Aoyama H.
Institución
Resumen
Pseudokirchneriella subcapitata is a unicellular green algae widely distributed in freshwater and soils. Due to its cosmopolitan characteristic, its use is recommended by national and international protocols in ecotoxicity studies. The alteration of phosphatase activities by agriculture pollutants like heavy metals has been extensively used as a biomarker in risk assessment and biomonitoring. In this study, we compared the extraction of acid phosphatase from P. subcapitata by different procedures and we studied the stability, substrates specificity, kinetics and the effect of Hg2+ in the crude extract. The freezing and thawing technique associated with probe sonication was the most suitable method of extraction. The enzyme was stable when frozen at -20°C for at least six months, showed an optimum pH of 5 and a Km value of 0.27 mM for p-nitrophenylphosphate (pNPP) as substrate. Some natural organic substrates were cleaved by a similar extent as the synthetic substrate pNPP. 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