Bovine respiratory syncytial virus (BRSV) causes pneumonia in young cattle. Modified-live-virus (MLV) and inactivated vaccines are currently used for the control of clinical effects of BRSV infections in cattle. On the present research, the stimulation of specific anti-BRSV immunoglobulin isotypes was investigated, through the use of different commercially available adjuvants (Water-in-oil emulsion, Quil A, Aluminumhydroxide) in inbred mice (Balb/C and C57BL/6). BRSV antibodies were measured using an enzyme-linked immunosorbent assay (ELISA) and the results were compared to the antibody levels induced by immunization of animals using live-BRSV-virus. Water-in-oil emulsion and alumadjuvant preparations induced higher levels of IgG1 immunoglobulins, whereas Quil A favored the production of IgG2 antibodies, this last being a more appropriate response profile for the specific case of BRSV. Not using adjuvants resulted in poor levels of specific antibodies. The isotype profile of specific antibodies obtained varied greatly depending on the adjuvants used. This information may be useful for the formulation of more effective BRSV inactivated vaccines; however, these findings have to be confirmed in cattle.401123322337Ayres, M., BioEstat 4.0: Aplicações estatísticas nas áreas das ciências biológicas e médicas (2005) Belém: Sociedade Civil Mamirauá, p. 290Corteling, R., Trifilieff, A., Gender comparison in a murine model of allergen-driven airway inflammation and the response to budesonide treatment BMC Pharmacology, 4, p. 4. , http://dx.crossref.org/10.1186%2F1471-2210-4-4, Available from, Accessed: Mar. 22, 2010. doi:10.1186/1471-2210-4-4Cox, J.C., Coulter, A.R., Adjuvants - a classification and review of their modes of action (1997) Vaccine, 15, pp. 248-256. , http://dx.doi.org/10.1016/S0264-410X(96)00183-1, Available from, Accessed: Mar. 01, 2008. doi: 10.1016/S0264-410X(96)00183-1Ellis, J., Efficacy of an inactivated respiratory syncytial virus vaccine in calves (2001) Journal of American Veterinary Medical Association, 230, pp. 1973-1980. , http://dx.doi.org/abs/10.2460/javma.2001.218.1973, Available from, Accessed: Mar. 01, 2008. doi: 10.2460/javma.2001.218.1973Ellis, J., Bovine respiratory syncytial virus-specific immune responses in cattle following immunization with modified-live and inactivated vaccines. Analysis of the specificity and activity of serum antibodies (1995) Veterinary Immunology and Immunopathology, 34, pp. 35-45. , http://dx.doi.org/10.1016/0165-2427(92)90150-O, Available from, Accessed: Jun. 06, 2009. doi: 10.1016/0165-2427(92)90150-OEllis, J., Efficacy of a saponin-adjuvanted inactivated respiratory syncytial virus vaccine in calves (2005) Canadian Veterinary Journal, 46, pp. 155-162. , http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1082864/, Available from, Accessed: Abr. 20, 2010Flores, E.F., Donis, R.O., Isolation of a mutant MDBK cell line resistant to bovine viral diarrhea virus infection due to a block in viral entry (1995) Virology, 208, pp. 565-575. , http://dx.doi.org/10.1006/viro.1995.1187, Available from, Accessed: Mar. 01, 2008. doi: 10.1006/viro.1995.1187Gershwin, L.J., Bovine respiratory syncytial virus infection: Immunopathogenic mechanisms (2007) Animal Health Resesearch Reviews, 8, pp. 207-213. , http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1660948, Available from, Accessed: Abr. 20, 2010. doi: 10.1017/S1466252307001405Gershwin, L.J., Immune mechanisms of pathogenetic synergy in concurrent bovine pulmonary infection with Haemophilus somnus and bovine respiratory syncytial virus (2005) Veterinary Immunology and Immunopathology, 107, pp. 119-130. , http://dx.doi.org/10.1016/j.vetimm.2005.04.004Hägglund, S., Bovine respiratory syncytial virus ISCOMs - protection in the presence of maternal antibodies (2004) Vaccine, 23, pp. 646-655. , http://dx.doi.org/10.1016/j.vaccine.2004.07.006, Available from, Accessed: Jun. 06, 2009. doi: 10.1016/j.vetimm.2005.04.004Kalina, W.V., Formalin-inactivated bovine RSV vaccine enhances a Th2 mediated immune response in infected cattle (2004) Vaccine, 22, pp. 1465-1474. , http://dx.doi.org/10.1016/j.vaccine.2003.10.024, Available from, Accessed: Abr. 20, 2010. doi: 10.1016/j.vaccine.2003.10.024Kerkhofs, P., et al, Immune responses to bovine respiratory syncytial virus (BRSV) following use of an inactivated BRSVPI3-Mannheimia haemolytica vaccine and a modified live BRSV-BVDV vaccine (2004) Veterinary Journal, 167, pp. 208-210. , http://dx.doi.org/10.1016/S1090-0233(03)00078-9, Available from, Accessed: Jun. 06, 2009. doi: 10.1016/S1090-0233(03)00078-9Kim, H.W., Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine (1969) American Journal of Epidemiology, 89, pp. 422-434. , http://aje.oxfordjournals.org/content/89/4/422.full.pdf+html, Available from, Accessed: Mar. 01Larsen, L.E., Bovine respiratory syncytial virus (BRSV) pneumonia in beef calf herds despite vaccination (2001) Acta Veterinaria Scandinavica, 42, pp. 113-121. , http://dx.crossref.org/10.1186%2F1751-0147-42-113, Available from, Accessed: Mar. 01, 2008. doi:10.1186/1751-0147-42-113Letellier, C., Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology (2008) Vaccine, 26, pp. 4840-4848. , http://dx.doi.org/10.1016/j.vaccine.2008.06.100, Available from, Accessed: Abr. 20, 2010. doi: 10.1016/j.vaccine.2008.06.100Mawhinney, I.C., Burrows, M.R., Protection against bovine respiratory syncytial virus challenge following a single dose of vaccine in young calves with maternal antibody (2005) Veterinary Record, 156, pp. 139-143. , http://veterinaryrecord.bvapublications.com/cgi/reprint/156/5/139, Available from, Accessed: Mar. 01, 2008Patel, J.R., Didlick, S.A., Evaluation of efficacy of an inactivated vaccine against bovine respiratory syncytial virus in calves with maternal antibodies (2004) American Journal of Veterinary Research, 65, pp. 417-421. , http://dx.doi.org/10.2460/ajvr.2004.65.417, Available from, Accessed: Mar. 01, 2008. doi: 10.2460/ajvr.2004.65.417Romera, S.A., Adjuvant effects of sulfolipo-cyclodextrin in a squalane-in-water and water-in-mineral oil emulsions for BHV-1 vaccines in cattle (2000) Vaccine, 19, pp. 132-141. , http://dx.doi.org/10.1016/S0264-410X(00)00104-3, Available from, Accessed: Mar. 01, 2008. doi: 10.1016/S0264-410X(00)00104-3Schreiber, P., High mortality rate associated with bovine respiratory syncytial virus (BRSV) infection in Belgian white blue calves previously vaccinated with an inactivated BRSV vaccine (2000) Journal Veterinary Medicine Series B, 47, pp. 535-550. , http://dx.doi.org/10.1046/j.1439-0450.2000.00380.x, Available from, Accessed: Mar. 01, 2008. doi: 10.1046/j.1439-0450.2000.00380.xSpilki, F.R., Susceptibility of different cell lines to infection with bovine respiratory syncytial virus Journal of Virological Methods, 131, pp. 130-133. , http://dx.doi.org/10.1016/j.jviromet.2005.08.002, Available from, Accessed: Mar. 01, 2008. doi: 10.1016/j.jviromet.2005.08.002Spilki, F.R., Effects of experimental inoculation of bovine respiratory syncytial virus in different inbred mice lineages: Establishment of a murine model for BRSV infection (2006) Veterinary Microbiology, 118, pp. 161-168. , http://dx.doi.org/10.1016/j.vetmic.2006.07.011, Available from, Accessed: Mar. 01, 2008. doi: 10.1016/j.vetmic.2006.07.011West, K., The effect of formalin-inactivated vaccine on respiratory disease associated with bovine respiratory syncytial virus infection in calves (1999) Vaccine, 17, pp. 809-820. , http://dx.doi.org/10.1016/S0264-410X(98)00265-5, Available from, Accessed: Mar. 01, 2008. doi: 10.1016/S0264-410X(98)00265-5