Artículos de revistas
Microrna Expression Profile In Epilepsy: Breaking Molecular Barriers
Registro en:
Journal Of Epilepsy And Clinical Neurophysiology. , v. 18, n. 2, p. 57 - 59, 2012.
16762649
10.1590/S1676-26492012000200008
2-s2.0-84872513628
Autor
Dogini D.B.
Avansini S.H.
Torres F.R.
Rogerio F.
Rocha C.S.
Secolin R.
Yasuda C.L.
Coan A.C.
Costa A.F.
Piaza A.C.S.
Reis L.A.M.R.
Queiroz L.D.S.
Tedeschi H.
Oliveira E.
Cendes F.
Lopes-Cendes I.
Institución
Resumen
Background: MicroRNAs (miRNAs) are small RNA molecules (21-24 nt) that negatively regulate gene expression, either by repression of translation or by degradation of messenger RNA. These molecules are involved in many important processes including cell differentiation, neurogenesis, formation of nervous system and others. Mesial temporal lobe epilepsy and epilepsy caused by cortical dysgenesis are among the leading causes of drug resistant epilepsy. Objectives: The objectives of this study were to characterize the expression profile of miRNAs and to investigate their regulation in mesial temporal lobe epilepsy (MTL) and in focal cortical dysplasias (FCDs). Methods: Total RNA was extracted from hippocampal and neocortical tissue, maintained in paraffin or fresh-frozen, from patients who underwent surgery for seizure control. For comparison we used tissue obtained from autopsy. RNA was extracted and used in real time PCR reactions (157 miRNAs analyzed) or microarray chips (847 miRNAs analyzed). Results: Bioinformatics analyzes identified three miRNAs with expression significantly different in patients with MTLE: let-7d, miR-29b and miR-30d; while in patients with FCDs we found 23 microRNAs differentially expressed. In addition, we found that different pathological forms of had different molecular signatures. Conclusions: The possible genes regulated by miRNAs with differential expression in tissue with mesial temporal sclerosis (MTS) are mainly related to neurogenesis and apoptosis. While in DCFs they were predominantly related to cell proliferation and migration. Our results demonstrate the importance of miRNA regulation the in molecular processes that lead to the lesions present in the MTS and the FCDs. 18 2 57 59 Bartel, D.P., MicroRNAs: Genomics, biogenesis, mechanism and functions (2004) Cell, 23, pp. 281-297 Bian, S., Sun, T., Functions of noncoding RNAs in neural development and neurological diseases (2011) Mol Neurobiol, 44, pp. 359-373 Chow, J.M., Shen, S.C., Wu, C.Y., Chen, Y.C., 12-o-Tetradecanoylphorbol 13-acetate prevents baicalein-induced apoptosis via activation of protein kinase C and JNKs in human leukemia cells (2006) Apoptosis, 11 (11), pp. 1999-2011. , Nov Dogini, D.B., Ribeiro, P.A., Rocha, C., Pereira, T.C., Lopes-Cendes, I., MicroRNA expression profile in murine central nervous system development (2008) J Mol Neurosci, 35, pp. 331-337 Guerrini, R., Doyns, W.B., Barkovich, A.J., Abnormal development of the human cerebral cortex: Genetics, functional consequences and treatment options (2008) Trends in Neuroscience, 31, pp. 154-162 Weng, H., Shen, C., Hirokawa, G., Ji, X., Takahashi, R., Shimada, K., Kishimoto, C., Iwai, N., Plasma miR-124 as a biomarker for cerebral infarction (2011) Biomedical Research, 32, pp. 135-141 Hui, C., How, E.I., Liu, F.-F., Micro-RNAs as diagnostic or prognostic markers in human epithelial malignancies (2011) BMC Cancer, 11, p. 500 Lewis, B.P., Burge, C.B., Bartel, D.P., Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets (2005) Cell, 120, pp. 15-20 Mathern, G.W., Challenges in the surgical treatment of epilepsy patients with cortical dysplasia (2009) Epilepsia, 50, pp. 45-50 Olive, V., Jiang, I., He, L., Mir-17-92, a cluster of miRNAs in the midst of the cancer network (2010) The International Journal of Biochemistry & Cell Biology, 42, pp. 1348-1354 Shen, Q., Temple, S., Fine control: MicroRNA regulation of adult neurogenesis (2009) Nature Neuroscience, 12, pp. 369-370 Shi, Y., Zhao, X., Hsieh, J., Wichterle, H., Impey, S., Banerjee, S., Neveu, P., Kosik, K.S., MicroRNA regulation of neural stem cells and neurogenesis (2012) J Neurosci, 10-30 (45), pp. 14931-14936 Vo, N., Klein, M.E., Varlamova, O., Keller, D.M., Yamamoto, T., Goodman, R.H., Impey, S., A cAMP-response element binding protein-induced microRNA regulates neuronal morphogenesis (2005) Proc Natl Acad Sci USA, 102, pp. 16426-16431 Wieser, H.G., ILAE Commission Report. Mesial temporal lobe epilepsy with hippocampal sclerosis (2004) Epilepsia, 45, pp. 695-714 Zhang, Y.K., Wang, H., Leng, Y., Li, Z.L., Yang, Y.F., Xiao, F.J., Li, Q.F., Wang, L.S., Biochem. Overexpression of microRNA-29b induces apoptosis of multiple myeloma cells through down regulating Mcl-1 (2011) Biophys Res Commun, 414 (1), pp. 233-239. , Oct