Artículos de revistas
Identification Of Corynebacterium Spp. Isolated From Bovine Intramammary Infections By Matrix-assisted Laser Desorption Ionization-time Of Flight Mass Spectrometry
Registro en:
Veterinary Microbiology. Elsevier, v. 173, n. 01/02/15, p. 147 - 151, 2014.
3781135
10.1016/j.vetmic.2014.06.028
2-s2.0-84906790543
Autor
Goncalves J.L.
Tomazi T.
Barreiro J.R.
Braga P.A.D.C.
Ferreira C.R.
Araujo Junior J.P.
Eberlin M.N.
dos Santos M.V.
Institución
Resumen
Corynebacterium species (spp.) are among the most frequently isolated pathogens associated with subclinical mastitis in dairy cows. However, simple, fast, and reliable methods for the identification of species of the genus Corynebacterium are not currently available. This study aimed to evaluate the usefulness of matrix-assisted laser desorption ionization/mass spectrometry (MALDI-TOF MS) for identifying Corynebacterium spp. isolated from the mammary glands of dairy cows. Corynebacterium spp. were isolated from milk samples via microbiological culture (n=180) and were analyzed by MALDI-TOF MS and 16S rRNA gene sequencing. Using MALDI-TOF MS methodology, 161 Corynebacterium spp. isolates (89.4%) were correctly identified at the species level, whereas 12 isolates (6.7%) were identified at the genus level. Most isolates that were identified at the species level with 16 S rRNA gene sequencing were identified as Corynebacterium bovis (n=156; 86.7%) were also identified as C. bovis with MALDI-TOF MS. Five Corynebacterium spp. isolates (2.8%) were not correctly identified at the species level with MALDI-TOF MS and 2 isolates (1.1%) were considered unidentified because despite having MALDI-TOF MS scores >2, only the genus level was correctly identified. Therefore, MALDI-TOF MS could serve as an alternative method for species-level diagnoses of bovine intramammary infections caused by Corynebacterium spp. © 2014 Elsevier B.V. 173 01/02/15 147 151 Alatoom, A.A., Cazanave, C.J., Cunningham, S.A., Ihde, S.M., Patel, R., Identification of non-diphtheriae Corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (2012) J. Clin. Microbiol., 50, pp. 160-163 Barreiro, J.R., Ferreira, C.R., Sanvido, G.B., Kostrzewa, M., Maier, T., Wegemann, B., Böttcher, V., dos Santos, M.V., Short communication: Identification of subclinical cow mastitis pathogens in milk by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (2010) J. Dairy Sci., 93, pp. 5661-5667 Braga, P.A.C., Tata, A., Goncalves dos Santos, V., Barreiro, J.R., Schwab, N.V., Veiga dos Santos, M., Eberlin, M.N., Ferreira, C.R., Bacterial identification: from the agar plate to the mass spectrometer (2013) RSC Adv., 3, pp. 994-1008 Coyle, M.B., Lipsky, B.A., Coryneform bacteria in infectious-diseases-clinical and laboratory aspects (1990) Clin. Microbiol. Rev., 3, pp. 227-246 Halasa, T., Huijps, K., Osteras, O., Hogeveen, H., Economic effects of bovine mastitis and mastitis management: a review (2007) Vet. Q., 29, pp. 18-31 Haltia, L., Honkanen-Buzalski, T., Spiridonova, I., Olkonen, A., Myllys, V., A study of bovine mastitis, milking procedures and management practices on 25 Estonian dairy herds (2006) Acta Vet. Scand., 48, p. 22 Hogan, J.S., González, R.S., Harmon, R.J., Nickerson, S.C., Oliver, S.P., Pankey, J.W., Smith, K.L., Laboratory handbook on bovine mastitis (1999) A Global Organization for Mastitis Control and Milk Quality, , National Mastitis Council Inc., Madison, WI, pp. 1-39, 135-136 Huxley, J.N., Helps, C.R., Bradley, A.J., Identification of Corynebacterium bovis by endonuclease restriction analysis of the 16S rRNA gene sequence (2004) J. Dairy Sci., 87, pp. 38-45 Konrad, R., Berger, A., Huber, I., Boschert, V., Hörmansdorfer, S., Busch, U., Hogardt, M., Sing, A., Matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry as a tool for rapid diagnosis of potentially toxigenic Corynebacterium species in the laboratory management of diphtheria-associated bacteria (2010) Euro Surveill., 15, pp. 9-13 Maier, T., Klepel, S., Renner, U., Kostrzewa, M., Fast and reliable MALDI-TOF MS-based microorganism identification (2006) Nature Methods, , Nature Publishing Group, Leipzig, Germany, Application note Oliver, S.P.O., González, R.N., Hogan, J.S., Jayarao, B.M., Owens, W.E., Microbiological procedures for the diagnosis of bovine udder infection and determination of milk quality (2004) A Global Organization for Mastitis Control and Milk Quality, , National Mastitis Council Inc., Verona, WI, pp. 1-40, 44-46 Pascual, C., Lawson, P.A., Farrow, J.A., Gimenez, M.N., Collins, M.D., Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences (1995) Int. J. Syst. Bacteriol., 45, pp. 724-728 Ryzhov, V., Fenselau, C., Characterization of the protein subset desorbed by MALDI from whole bacterial cells (2001) Anal. Chem., 73, pp. 746-750 Schukken, Y.H., Gonzalez, R.N., Tikofsky, L.L., Schulte, H.F., Santisteban, C.G., Welcome, F.L., Bennett, G.J., Zadoks, R.N., CNS mastitis: nothing to worry about? (2009) Vet. Microbiol., 134, pp. 9-14 Theel, E.S., Schmitt, B.H., Hall, L., Cunningham, S.A., Walchak, R.C., Patel, R., Wengenack, N.L., Formic acid-based direct on-plate testing of yeast and Corynebacterium species by Bruker biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry (2012) J. Clin. Microbiol., 50, pp. 3093-3095 Vaneechoutte, M., Riegel, P., Debriel, D., Monteil, H., Verschraegen, G., Derouck, A., Claeys, G., Evaluation of the applicability of amplified rDNA-restriction analysis (ARDRA) to identification of species of the genus Corynebacterium (1995) Res. Microbiol., 146, pp. 633-641 Vila, J., Juiz, P., Salas, C., Almela, M., de la Fuente, C.G., Zboromyrska, Y., Navas, J., Martinez-Martinez, L., Identification of clinically relevant Corynebacterium spp., Arcanobacterium haemolyticum, and Rhodococcus equi by matrix-assisted laser desorption ionization-time of flight mass spectrometry (2012) J. Clin. Microbiol., 50, pp. 1745-1747 Watts, J.L., Lowery, D.E., Teel, J.F., Rossbach, S., Identification of Corynebacterium bovis and other coryneforms isolated from bovine mammary glands (2000) J. Dairy Sci., 83, pp. 2373-2379 Welker, M., Proteomics for routine identification of microorganisms (2011) Proteomics, 11, pp. 3143-3153