Artículos de revistas
Fourier Transform Infrared Microspectroscopy As A Bacterial Source Tracking Tool To Discriminate Fecal E. Coli Strains
Registro en:
Microchemical Journal. , v. 99, n. 1, p. 15 - 19, 2011.
0026265X
10.1016/j.microc.2011.03.002
2-s2.0-79957632533
Autor
Carlos C.
Maretto D.A.
Poppi R.J.
Sato M.I.Z.
Ottoboni L.M.M.
Institución
Resumen
The aim of this work was to use the FT-IR microspectroscopy technique, a union of a FT-IR spectrometer with a microscope, to discriminate fecal Escherichia coli strains from cows, chickens and humans and to compare the efficiency of this method with the genomic fingerprinting method, BOX-PCR. The obtained BOX-PCR profiles were able to correctly discriminate 93.75% of the chicken strains, 80% of the cow strains and 65% of the human strains. An efficient PLS-DA model was developed, using orthogonal signal correction and the second derivate of the FT-IR spectra. This model allowed the correct discrimination, according to the animal source, of all the E. coli strains analyzed. The bands in the FT-IR spectra that were responsible for the strains discrimination were in the region between 2816 and 3026cm -1 wavenumber, described as fatty acids. 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