Artículos de revistas
Environmental Contamination And Enterotoxigenic Profile Of Bacillus Cereus Isolated In Food Services [contaminação Ambiental E Perfil Toxigênico De Bacillus Cereus Isolados Em Serviços De Alimentação]
Registro en:
Ciencia Rural. , v. 38, n. 2, p. 504 - 510, 2008.
1038478
10.1590/S0103-84782008000200033
2-s2.0-41149105307
Autor
Soares C.M.
Valadares G.F.
De Azeredo R.M.C.
Kuaye A.Y.
Institución
Resumen
Ninety air samples and ninety six samples from benches and equipments surfaces were collected in two food services for investigation of Bacillus cereus contamination sources and characterization of strains toxin profiles. B. cereus was detected in 84.4% and 44.8% from air samples and samples from benches and equipments surfaces, respectively. The potential of enterotoxin production was investigated using polymerase chain reaction (PCR) methods for genes hblA, hblD e hblC (encoding hemolysin BL) and for genes nheA, nheB and nheC (encoding non-hemolytic enterotoxin - NHE). From 70 isolates investigated 14.3% were positive for the three HBL encoding genes and 12.8% were positive for the three NHE encoding genes. The Bacillus Diarrhoeal Enterotoxin Visual Immunoassay (BDE-VIA; Tecra) also was used for NHE detection. The results obtained with BDE-VIA revealed that 61.4% from the 70 strains are NHE producers. 38 2 504 510 ANDERSSON, A., What problems does the food industry have the spore-forming pathogens Bacillus cereus and Clostridium perfrigens? (1995) International Journal of Food Microbiology, 28 (2), pp. 145-155 BEECHER, D.J., MACMILLAN, J.D., Characterization of the components of hemolysin BL from Bacillus cereus (1991) Infection and Immunity, 59 (5), pp. 1778-1784 BEECHER, D.J., Enterotoxic activity of hemolysin BL from Bacillus cereus (1995) Infection and Immunity, 63 (11), pp. 4423-4428 EVANCHO et al. Microbiological monitoring of the food processing environment. In: DOWNES, F.P. ITO, K. Compendium of methods for the microbiological examination of foods. 4.ed. Washington, DC: American Public Health Association, 2001. cap.3, p.25-35GHELARDI, E., Identification and characterization of toxigenic B. cereus isolates responsible for two food-poisoning outbreaks (2002) FEMS Microbiology Letters, 208 (1), pp. 129-134 GRANUM, P.E. Bacillus cereus and its toxins. Journal of Applied Bacteriology, Symposium Supplement, n.76, p.61S-66S, 1994GRANUM, P.E., Bacillus cereus (1997) Food microbiology: Fundamentals and frontiers, pp. 327-336. , DOYLE, M.P. et al, Washington, DC: ASM GRANUM, P.E., The sequence of the non-haemolytic enterotoxin operon from Bacillus cereus (1999) FEMS Microbiology Letters, 177 (2), pp. 225-229 GUINEBRETIERE, M.H., NGUYEN-THE, C. Sources of Bacillus cereus contamination in a pasteurised zucchini purée processing line, differentiated by two PCR-based methods (2003) FEMS Microbiology Ecology, 43 (2), pp. 207-215 HANSEN, B.M., HENDRIKSEN, N.B., Detection of enterotoxin Bacillu cereus and Bacillus thuringiensis strains by PCR analysis (2001) Applied and Environmental Microbiology, 67 (1), pp. 185-189 HEINRICHS, A.H., Molecular cloning and characterization of the hblA gene encoding the B component of hemolysin BL from Bacillus cereus (1993) Journal Bacteriology, 175, pp. 6760-6766 KOTIRANTA, A., Epidemiology and pathogenesis of Bacillus cereus infections (2000) Microbes and Infection, 2 (2), pp. 189-198 KRAMER, J.M. GILBERT, R.J. Bacillus cereus and other Bacillus species. In: DOYLE, M.P. Food borne bacteria pathogens. New York: Marcel Dekker, 1989. p.21-69LUND, T., GRANUM, P.E., Characterisation of a nonhaemolytic enterotoxin complex from Bacillus cereus isolated after a food borne outbreak (1996) FEMS Microbiology Letters, 141 (2-3), pp. 151-156 LUND, T., GRANUM, P.E., Comparison of biological effect of the two different enterotoxin complexes isolated from three different strains of Bacillus cereus (1997) Microbiology, 143 (PART.10), pp. 3329-3336. , Reading, England, v PHELPS, R.J., MCKILLIP, J.L., Enterotoxin production in natural isolates of Bacillaceae outside the Bacillus cereus group (2002) Applied and Environmental Microbiology, 68 (6), pp. 3147-3151 RHODEHAMEL, E.J. HARMON, S.M. Bacillus cereus. In: FOOD AND DRUG ADMINISTRATION. Bacteriological analytical manual. 8.ed. Arlington: Association of Official Analytical Chemists International, 1998. cap.14, p.14.01-14.08RYAN, P.A., Molecular cloning and characterization of the genes encoding the L1 and L2 components of hemolysin BL from Bacillus cereus (1997) Journal Bacteriology, 179 (8), pp. 2551-2556 SAMBROOK, J., (1989) Molecular clonning: A laboratory manual, , 2.ed. New York: Cold Spring Harbor Laboratory, 120p SHARIF, F.A., ALAEDDINOGLU, G.A., Rapid and simple method for staining of the crystal protein of Bacillus thuringiensis (1988) Journal of Industrial Microbiology, 3, pp. 227-229