Artículos de revistas
Nitric Oxid Release May Be Involved In The Microcirculatory Response To Acetylcholine
Registro en:
European Journal Of Pharmacology. , v. 182, n. 1, p. 143 - 147, 1990.
142999
10.1016/0014-2999(90)90503-X
2-s2.0-0025294212
Autor
Fortes Z.B.
Oliveira M.A.
Scivoletto R.
Carvalho M.H.C.
De Nucci G.
Nigro D.
Institución
Resumen
An image-splitter television microscope for measurement of microvascular dimension changes in the rat exteriorized mesentery was used to investigate the role of endothelium-derived relaxing factor (EDRF)/nitric oxide (NO) in the microcirculation. This was done by studying the effect of NG-monomethyl-L-arginine (L-NMMA), a specific inhibitor of NO formation, on the responses induced by acetylcholine (an endothelium-dependent vasodilator agent) and sodium nitroprusside (an endothelium-independent vasodilator agent). The effect of acetylcholine on mesenteric A2 arterioles was impaired by previous application of L- but not D-NMMA to the preparations whereas the vasodilator response to sodium nitroprusside was not altered. The effect of L-NMMA was slow to disappear, unless accelerated by a 3-fold molar excess of L- but not D-arginine. It is suggested that EDRF/NO might be involved in the vasodilator response to endothelium-dependent agents such as acetylcholine at the microcirculatory level and that L-arginine might be the physiological precursor of NO. © 1990. 182 1 143 147 Amezcua, Palmer, De Souza, Moncada, Nitric oxide synthesized from L-arginine regulates vascular tone in the coronary circulation of the rabbit (1989) Br. J. Pharmacol., 97, p. 1119 Baez, Recording of microvascular dimensions with an image-splitter television microscope (1966) J. Appl. Physiol., 21, p. 299 Baez, Simultaneous measurements of radii and wall thickness of microvessels in the anesthetized rat (1969) Circ. Res., 25, p. 315 Carvalho, Furchgott, Vasodilation of the rabbit mesenteric vascular bed by acetylcholine and A 23187 (1981) The Pharmacologist, 23, p. 223 Fortes, Garcia-Leme, Scivoletto, Influence of diabetes on the reactivity of mesenteric microvessels to histamine, bradykinin and acetylcholine (1983) Br. J. Pharmacol., 78, p. 39 Furchgott, Carvalho, Khan, Matsunaga, Evidence for endothelium-dependent vasodilation of resistance vessels by acetylcholine (1987) Blood Vessels, 24, p. 145 Gore, Bohlen, Microvascular pressures in rat intestinal muscle and mucosal villi (1977) Am. J. Physiol., 233, p. H685 Ignarro, Gold, Wood, Byrns, Buga, Chaudhuri, Alteration of endothelium-dependent arterial relaxation by arginine analogs and arginine depletion (1989) J. Vasc. Med. Biol., 1, p. 94 Kaley, Koller, Cherry, Messina, Wolin, Control of microvascular tone by EDRF and prostaglandins (1989) J. Vasc. Med. Biol., 1, p. 95 Katusic, Vanhoutte, Endothelium-dependent contractions to NG-monomethyl-L-arginine in the canine basilar artery (1989) J. Vasc. Med. Biol., 1, p. 95 Palmer, Ashton, Moncada, Vascular endothelial cells synthesize nitric oxide from L-arginine (1988) Nature, 333, p. 664 Palmer, Ferrige, Moncada, Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor (1987) Nature, 327, p. 524 Palmer, Rees, Ashton, Moncada, L-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation (1988) Biochem. Biophys. Res. Commun., 153, p. 1251 Rees, Palmer, Hodson, Moncada, A specific inhibition of nitric oxide formation from L-arginine attenuates endothelium-dependent relaxation (1989) Br. J. Pharmacol., 96, p. 418 Rees, Palmer, Moncada, Role of endothelium-derived nitric oxide in the regulation of blood pressure (1989) Proc. Natl. Acad. Sci. U.S.A., 86, p. 3375 Zweifach, Indirect methods for regional blood flow I Microscopic observation of circulation in rat mesoappendix and dog omentum Use in study of vasotropic substances (1948) Methods Med Res, 1, p. 131