Actas de congresos
The Effects Of Copper Ions On The Synthesis Of Periplasmic And Membrane Proteins In Acidithiobacillus Ferrooxidans As Analyzed By Sds-page And 2d-page
Registro en:
Hydrometallurgy. , v. 71, n. 01/02/15, p. 165 - 171, 2003.
0304386X
10.1016/S0304-386X(03)00153-1
2-s2.0-17944384988
Autor
Felicio A.P.
Garcia Jr. O.
Bertolini M.C.
Ottoboni L.M.M.
Novo M.T.M.
Institución
Resumen
The effects of 200 mM copper ions on the synthesis of membrane and periplasmic proteins were investigated in iron-grown cells of Acidithiobacillus ferrooxidans (At. ferrooxidans). Total membrane protein profiles of cells grown in the absence of copper ions (unadapted cells) and in the presence of copper ions (copper-adapted cells) were compared by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Crude preparations of outer membrane and periplasmic proteins were analyzed by SDS-PAGE. The synthesis of proteins was diminished or increased in the presence of copper ions. Low molecular weight proteins (<14 kDa) were significantly repressed by copper. These proteins are probably acidic proteins located in the outer membrane. 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