Artículos de revistas
Purification And Characterization Of An N-acetylglucosamine-binding Lectin From Koelreuteria Paniculata Seeds And Its Effect On The Larval Development Of Callosobruchus Maculatus (coleoptera: Bruchidae) And Anagasta Kuehniella (lepidoptera: Pyralidae)
Registro en:
Journal Of Agricultural And Food Chemistry. , v. 51, n. 10, p. 2980 - 2986, 2003.
218561
10.1021/jf034013i
2-s2.0-0038746856
Autor
Macedo M.L.R.
Damico D.C.S.
Freire M.D.G.M.
Toyama M.H.
Marangoni S.
Novello J.C.
Institución
Resumen
This study describes the purification of an N-acetylglucosamine-binding lectin from Koelreuteria paniculata seeds and its effects on the larval development of Callobruchus maculatus and Anagasta kuehniella. The lectin (KpLec) was characterized and isolated by gel filtration, affinity column, and reverse phase chromatography. SDS-PAGE indicated that this lectin is a dimer composed of subunits of 22 and 44 kDa. The N terminus exhibited 40% similarity with Urtiga dioica agglutinin. KpLec was tested for anti-insect activity against C. maculatus and A. kuehniella. With regard to C. maculatus, an artificial diet containing 0.7 and 1% KpLec produced LD 50 and ED 50 value, respectively. However, for A. kuenhiella, an artificial diet containing 0.65% KpLec produced an LD 50, whereas 0.2% KpLec produced an ED 50. The transformation of genes coding for this lectin could be useful in the development of insect resistance in important agricultural crops. 51 10 2980 2986 Singh, B.B., Singh, S.R., Adjadi, O., Bruchid resistance in cowpea (1985) Crop Sci., 25, pp. 736-739 Latunde-Data, A.O., Genetic manipulation of the cowpea (Vigna unguiculata (L.) Walp.) for enhanced resistance to fungal pathogens and insect pests (1990) Adv. Agron., 44, pp. 133-154 Hall, A.E., Singh, B.B., Ehlers, J.D., Cowpea breeding (1997) Plant Breed. Rev., 15, pp. 217-274 Shade, R.E., Murdock, L.L., Foard, D.E., Pomeroy, M.A., Artificial seed system for bioassay of cowpea weevil (Coleoptera: Bruchidae) growth and development (1986) Environ. Entomol., 15, pp. 1286-1291 Ebeling, W., Urban entomology (1996) Military Pest Management Handbook, 2nd Ed., , University of California, Division of Agricultural Sciences: Berkeley, CA Macedo, M.L.R., Fernandes, K.V.S., Sales, M.P., Xavier-Filho, J., Vicilin variants and the resistance of cowpea (Vigna unguiculata) seeds to the cowpea weevil (Callosobruchus maculatus) (1993) Comp. Biochem. Physiol., 105 C, pp. 89-94 Macedo, M.L.R., Fernandes, K.V.S., Sales, M.P., Xavier-Filho, J., Purification and properties of storage proteins (vicilins) from cowpea (Vigna unguiculata) seeds which are susceptible of resistance to the Bruchidae beetle Callosobruchus maculatus (1995) Braz. J. Med. Biol. Res., 28, pp. 183-189 Carlini, C.R., Oliveira, A.E.A., Azambuja, P., Xavier-Filho, J., Wells, M., A Biological effects of canatoxin in different insect models: Evidence for a proteolytic activation of the toxin by insect cathepsin-like enzymes (1997) J. Econ. Entomol., 90, pp. 340-348 Macedo, M.L.R., Coelho, M.B., Freire, M.G.M., Machado, O.L.T., Marangoni, S., Novello, J.C., Effect of a toxic protein isolated from Zen mays seeds on the development and survival of the cowpea weevil, Callosobruchus maculatus (2000) Protein Pept. Lett., 17, pp. 25-31 Macedo, M.L.R., Freire, M.G.M., Novello, J.C., Marangoni, S., Talisia esculenta lectin and larval development of Callosobruchus maculatus and Zabrotes subfasciatus (Coleoptera: Bruchidae) (2002) Biochim. Biophys Acta, 1571, pp. 83-88 Peumans, W.J., Van Damme, E.J.M., Plant lectins: Versatile proteins with important perspectives in biotechnology (1998) Biotechnol. Genet. Eng. Rev., 15, pp. 199-299 Roy, A., Banerjee, S., Majumder, P., Das, S., Efficiency of mannose-binding plant lectins in controlling a homopteran insect, the red cotton bug (2002) J. Agric. Food Chem., 50, pp. 6775-6779 Rahbé, Y., Sauvion, N., Febway, G., Pewmans, W.J., Gatehouse, A.M.R., Toxicity of lectins and processing of ingested proteins in the pea aphid, Acrythosiphon pisum (1995) Entomol. Exp. Appl., 76, pp. 143-155 Fitches, E., Gatehouse, A.M.R., Gatehouse, J.A., Effects of snowdrop lectin (GNA) delivered via artificial diet and in transgenic plants on the development of tomato moth (Lacanobia oleracea) larvae in laboratory and glasshouse trials (1997) J. Insect Physiol., 43, pp. 727-739 Gatehouse, A.M.R., Powell, K.S., Peumans, W.J., Van Damme, E.J.M., Gatehouse, J.A., Insecticidal properties of plant lectins Their potential in plant protection (1995) Lectins: Biomedical Perspectives, pp. 35-38. , Pusztai, A. J., Bardocz, S., Eds. Taylor and Francis: Hants, U.K Harper, M.S., Hopkins, T.L., Czapla, T.H., Effect of wheat germ agglutinin on formation and structure of the peritrophic membrane in European corn borer (Ostrinia nubilalis) larvae (1998) Tissue Cell, 30, pp. 166-176 Czapla, T.H., Plant lectin as insect control proteins in transgenic plants (1997) Advances in Insect Control: The Role of Transgenic Plants, pp. 123-138. , Carozzi, N., Koziel, M., Eds. Taylor and Francis: Basingstoke, U.K Bandyopahyay, S., Roy, A., Das, S., Binding of garlic (Allium sativum) leaf lectin to gut receptors of homopteran pests is correlated to its insecticidal activity (2001) Plant Sci., 16, pp. 1025-1033 Chrispeels, M.J., Raikhel, N.V., Lectins, lectin genes, and their role in plant defense (1991) Plant Cell, 3, pp. 1-9 Zhu, K., Huesing, J.E., Shade, R.E., Bressan, R.A., Hasegawa, P.M., Murdock, L.L., An insecticidal N-acetylglucosamine specific lectin gene from Griffonia simplificolia (Leguminosae) (1996) Plant Physiol., 110, pp. 195-202 Dubois, M., Gilles, K.A., Hamilton, J.L., Rebers, P.A., Smith, F., Colorimetric method for determination of sugars and related substances (1956) Anal. Chem., 28, pp. 350-356 Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding (1976) Anal. Biochem., 72, pp. 248-254 Henrikson, R.L., Meredith, S.C., Amino acid analysis by reverse phase high performance liquid chromatography: Pre-column derivatization with phenylisothiocyanate (1984) Anal. Biochem., 136, pp. 65-71 Laemmli, V.K., Cleavage of structural proteins during the assembly of the bacteriophage T4 (1970) Nature, 227, pp. 680-685 Ryan, C.A., Plant proteinases (1981) The Biochemistry of Plants, pp. 76-82. , Marcus, E., Ed. Academic Press: New York Sampietro, A.R., Isla, M.I., Quiroga, E.N., Vattuone, M.A., An N-acetylglucosamine oligomer binding agglutinin (lectin) from ripe Cyphomandra betacea Sendt (2001) Fruits Plant Sci., 160, pp. 659-667 Wang, H.X., Ng, T.B., Ooi, V.E.C., Studies on purification of a lectin from fruiting bodies of the edible shiitake mushroom Lentinus edodes (1999) Int. J. Biochem. Cell. Biol., 31, pp. 595-599 Broekaert, W.F., Van Parijs, J., Leyns, F., Ojos, H., Peumans, W.J., A chitin-binding lectin from stinging nettle rhizomes with antifungal properties (1989) Science, 245, pp. 1100-1102 Cavada, B.S., Santos, C.F., Granjeiros, T.B., Nunes, E.P., Sales, P.V.P., Ramos, R.L., Sousa, F.A.M., Calvete, J.J., Purification and characteriztion of a lectin from seeds of Vatairea macrocarpa Duke (1998) Phytochemistry, 49, pp. 675-680 Datta, P.K., Figueroa, M.O.R., Lajolo, F.M., Chemical modification and sugar binding properties of two major lectins from pinhão (Araucaria brasiliensis) seeds (1993) J. Agric. Food Chem., 41, pp. 1851-1855 Ray, S., Chatterjee, B.P., Saracin: A lectin from Saraca indica seed integument recognizes complex carbohydrates (1995) Phytochemistry, 3, pp. 643-649 Machuka, J.S., Okeola, O.G., Van Damme, Els.J.M., Chrispeels, M.J., Leuven, F.V., Peumans, W.J., Isolation and partial characterization of galactose-specific lectins from African yam beans, Sphenostyles stenocarpa Harms (1999) Phytochemistry, 51, pp. 721-728 Machuka, J.S., Van Damme, E.J.M., Peumans, W.J., Jackai, L.E.N., Effect of plant lectins on larval development of the legume pod borer, Maruca vitrata (1999) Entomol. Exp. Appl., 93, pp. 179-187 Murdock, L.L., Huesingh, J.E., Nielson, S.S., Pratt, R.C., Shade, R.E., Biologival effects of plant lectins on the cowpea weevil (1990) Phytochemistry, 29, pp. 85-89 Huesing, J.E., Murdock, L.L., Shade, R.E., Rice and stinging nettle lectins: Insecticidal activity similar to wheat germ agglutinin (1991) Phytochemistry, 30, pp. 3565-3568 Pusztai, A., Bardocz, S., Biological effects of plant lectins on the gastrointestinal tract: Metabolic consequences and applications (1996) Trends Glycosci. Glycotechnol., 8, pp. 149-165 Harper, M.S., Hopkins, T.L., Czapla, T.H., Effect of wheat germ agglutinin on formation and structure of the peritrophic membrane in European corn borer (Ostrinia nubilalis) larvae (1998) Tissue Cell, 30, pp. 166-176 Sales, M.P., Pimenta, P.P., Paes, N.S., Grossi-de-Sá, M.F., Xavier-Filho, J., Vicilins (7S storage globulins) of cowpea (Vigna unguiculata) seeds bind to chitinous structures of the midgut of Callosobruchus maculatus (Coleoptera: Bruchidae) larvae (2001) Brazil. J. Med. Biol. Res., 34, pp. 27-34 Czapla, T.H., Lang, B.A., Effect of plant lectins on the development of European corn borer (Lepidoptera: Pyralidae) and southern corn rootworm (Coleoptera: Chrysomelidae) (1990) J. Econ. Entomol., 6, pp. 2480-2485