Artículo
In vitro gas production of foliage from three browse tree species treated with different dose levels of exogenous fibrolytic enzymes
Autor
LOPEZ AGUIRRE, DANIEL; 266275
Vázquez Armijo, José Fernando; 44690
LOPEZ VILLALOBOS, NICOLAS; 65725
LEE RANGEL, HECTOR AARON; 131237
Zeidan Mohamed Salem, Abdelfattah; 274697
BORQUEZ GASTELUM, JOSE LUIS; 90207
Domínguez Vara, Ignacio Arturo; 121047
ROJO RUBIO, ROLANDO; 25090
LOPEZ AGUIRRE, DANIEL
Vázquez Armijo, José Fernando
LOPEZ VILLALOBOS, NICOLAS
LEE RANGEL, HECTOR AARON
Zeidan Mohamed Salem, Abdelfattah
BORQUEZ GASTELUM, JOSE LUIS
Domínguez Vara, Ignacio Arturo
ROJO RUBIO, ROLANDO
Institución
Resumen
The aim of this study was to evaluate the effect of different dose levels of exogenous fibrolytic enzymes (EFE) on in vitro ruminal fermentation kinetics and energy utilization of foliages from three browse trees (Pithecellobium dulce, Heliocarpus velutinus and Guazuma ulmifolia). Mixture of EFE product was added to the leaves of the three browse tree species at three dose levels: 0 (control), 3.5 and 7.0 mg/g of DM. Chemical composition of the foliages, including plant secondary metabolites such as total phenolics (TP), saponins (SAP) and aqueous fraction (AF), was determined. In addition, in vitro assaying of ruminal gas production kinetics was determined for the three browse three foliages treated with EFE. P. dulce had the highest crude protein content (p < 0.05), whereas G. ulmifolia had the highest content of neutral detergent fibre and SAP (p < 0.05) and H. velutinus had the lowest content of TP (p < 0.05). The interaction between tree species and dose level of EFE was significant (p < 0.05) for gas production (GP) at 24 h of incubation, parameters b and c of the accumulated GP curve, short-chain fatty acids (SCFA) and metabolizable energy (ME). The lowest (p < 0.01) extent of accumulated GP as well as the b and c values occurred in G. ulmifolia at 0 mg EFE/g DM. P. dulce had the highest (p < 0.05) values for ME and SCFA at the highest dose of EFE. Tree species and dose level had significant (p < 0.05) effects on all parameters describing in vitro ruminal fermentation kinetics and energy utilization. Addition of EFE improved the fermentation kinetics of the browse species considered in this study. UAEM