Fibroblast growth factor 17 and bone morphogenetic protein 15 enhance cumulus expansion and improve quality of in vitro-produced embryos in cattle

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMachado, Mariana Fernandes
dc.creatorCaixeta, Ester Siqueira
dc.creatorSudiman, Jaqueline
dc.creatorGilchrist, Robert B.
dc.creatorThompson, Jeremy G.
dc.creatorLima, Paula Fernanda
dc.creatorPrice, Christopher A.
dc.creatorBuratini, Jose
dc.date2015-10-21T21:20:58Z
dc.date2016-10-25T21:09:27Z
dc.date2015-10-21T21:20:58Z
dc.date2016-10-25T21:09:27Z
dc.date2015-08-01
dc.date.accessioned2017-04-06T09:10:50Z
dc.date.available2017-04-06T09:10:50Z
dc.identifierTheriogenology. New York: Elsevier Science Inc, v. 84, n. 3, p. 390-398, 2015.
dc.identifier0093-691X
dc.identifierhttp://hdl.handle.net/11449/129553
dc.identifierhttp://acervodigital.unesp.br/handle/11449/129553
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2015.03.031
dc.identifierWOS:000356318100010
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0093691X15001600
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/940108
dc.descriptionBone morphogenetic protein 15 (BMP15) and members of the fibroblast growth factor (FGF) family are expressed by the oocyte and are involved in the control of cumulus cell function. We tested the hypothesis that FGF17, alone or combined with BMP15 in the maturation medium, enhances cumulus expansion, meiosis progression, embryonic development, and expression of mRNA encoding key genes regulating expansion (prostaglandin-endoperoxide synthase 2 [PTGS2], hyaluronan synthase 2 [HAS2], tumor necrosis factor-stimulated gene 6 [TNFAIP6], and pentraxin 3 [PTX3]) and markers of oocyte developmental competence (phosphofructokinase [PFKP], gremlin [GREM1], versican [VCAN], and the genomic progesterone receptor [nPR]) in cumulus cells. Fibroblast growth factor 17 and BMP15 increased the percentage of fully expanded cumulus-oocyte complexes (COCs), but there was no additive effect when both were combined. Neither FGF17 nor BMP15 altered the percentage of oocytes reaching meiosis II at the end of COC culture or cleavage and blastocyst rates after IVF. However, embryo quality, as assessed by the number of cells in the inner cell mass, was improved by the combination of FGF17 with BMP15. Fibroblast growth factor 17 alone did not alter gene expression in cumulus cells at the end of IVM, whereas BMP15 increased PTGS2 and PTX3 mRNA levels. The combination of FGF17 and BMP15 increased nPR mRNA abundance in cumulus cells but did not change the expression of other markers of developmental competence. This study provides novel evidence that FGF17 enhances cumulus expansion in bovine COCs submitted to IVM and that the supplementation of the IVM medium with FGF17 and BMP15 may improve embryo quality.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherElsevier B.V.
dc.relationTheriogenology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectFibroblast growth factor 17
dc.subjectBone morphogenetic protein15
dc.subjectOocyte maturation
dc.subjectCumulus expansion
dc.subjectEmbryo quality
dc.titleFibroblast growth factor 17 and bone morphogenetic protein 15 enhance cumulus expansion and improve quality of in vitro-produced embryos in cattle
dc.typeOtro


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