Artigo de peri??dico
Fluorescent lifetime imaging microscopy using Europium complexes improves atherosclerotic plaques discrimination
Registro en:
1569-5794
10
32
10.1007/s10554-016-0936-7
45.040
Autor
SICCHIERI, LETICIA B.
NATAL, RODRIGO de A.
COURROL, LILIA C.
Resumen
The objective of this study is to characterize
arterial tissue with and without atherosclerosis by fluorescence
lifetime imaging microscopy (FLIM) using Europium
Chlortetracycline complex (EuCTc) as fluorescent marker.
For this study, twelve rabbits were randomly divided into
a control group (CG) and an experimental group (EG),
where they were fed a normal and hypercholesterolemic
diet, respectively, and were treated for 60 days. Cryosections
of the aortic arch specimens were cut in a vertical
plane, mounted on glass slides, and stained with Europium
(Eu), Chlortetracycline (CTc), Europium Chlortetracycline
(EuCTc), and Europium Chlortetracycline Magnesium
(EuCTcMg) solutions. FLIM images were obtained with
excitation at 405 nm. The average autofluorescence lifetime
within plaque depositions was ~1.36 ns. Reduced
plaque autofluorescence lifetimes of 0.23 and 0.31 ns were
observed on incubation with EuCTc and EuCTcMg respectively.
It was observed a quenching of collagen, cholesterol
and TG emission spectra increasing EuCTc concentration.
The drastic reduction in fluorescence lifetimes is due to
a resonant energy transfer between collagen, triglycerides,
cholesterol and europium complexes, quenching
fluorescence. Funda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (FAPESP) FAPESP: 08/57906-3, 14/06960-9