Effects of FSH on the expression of receptors for oocyte-secreted factors and members of the EGF-like family during in vitro maturation in cattle

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCaixeta, Ester Siqueira
dc.creatorMachado, Mariana Fernandes
dc.creatorRipamonte, Paula
dc.creatorPrice, Christopher
dc.creatorBuratini, José
dc.date2014-05-27T11:29:58Z
dc.date2016-10-25T18:51:33Z
dc.date2014-05-27T11:29:58Z
dc.date2016-10-25T18:51:33Z
dc.date2013-07-22
dc.date.accessioned2017-04-06T02:31:27Z
dc.date.available2017-04-06T02:31:27Z
dc.identifierReproduction, Fertility and Development, v. 25, n. 6, p. 890-899, 2013.
dc.identifier1031-3613
dc.identifier1448-5990
dc.identifierhttp://hdl.handle.net/11449/76016
dc.identifierhttp://acervodigital.unesp.br/handle/11449/76016
dc.identifier10.1071/RD12125
dc.identifierWOS:000321628100005
dc.identifier2-s2.0-84880241222
dc.identifierhttp://dx.doi.org/10.1071/RD12125
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896731
dc.descriptionFSH induces expansion of bovine cumulus-oocyte complexes (COCs) in cattle, which can be enhanced by oocyte-secreted factors (OSFs). In this study it was hypothesised that FSH stimulates COC expansion in part from direct stimulation of the epidermal growth factor (EGF)-like ligands amphiregulin (AREG), epiregulin (EREG) and betacellulin (BTC), but also in part through regulation of OSFs or their receptors in cumulus cells. Bovine COCs were cultured in defined medium with graded doses of FSH. In the absence of FSH, COCs did not expand. FSH caused cumulus expansion, and increased the abundance of AREG and EREG mRNA in a time- and dose-dependent manner, but decreased BTC mRNA levels. FSH had modest stimulatory effects on the levels of mRNA encoding the bone morphogenetic protein 15 (BMP15) receptor, BMPR1B, in cumulus cells, but did not alter mRNA expression of the growth and differentiation factor 9 (GDF9) receptor, TGFBR1. More interestingly, FSH dramatically stimulated levels of mRNA encoding two receptors for fibroblast growth factors (FGF), FGFR2C and FGFR3C, in cumulus cells. FSH also stimulated mRNA expression of FGFR1B, but not of FGFR2B in cumulus cells. Based on dose-response studies, FGFR3C was the receptor most sensitive to the influence of FSH. This study demonstrates that FSH stimulates the expression of EGF-like factors in bovine cumulus cells, and provides evidence that FSH differently regulates the expression of distinct receptors for OSFs in cumulus cells. © CSIRO 2013.
dc.languageeng
dc.relationReproduction, Fertility and Development
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcumulus expansion
dc.subjectEGF-like ligands
dc.subjectamphiregulin
dc.subjectbetacellulin
dc.subjectbone morphogenetic protein 15 receptor
dc.subjectbone morphogenetic protein receptor 1B
dc.subjectepiregulin
dc.subjectfibroblast growth factor receptor
dc.subjectfibroblast growth factor receptor 1B
dc.subjectfibroblast growth factor receptor 2c
dc.subjectfibroblast growth factor receptor 3c
dc.subjectfollitropin
dc.subjectgrowth differentiation factor 9
dc.subjectmessenger RNA
dc.subjectunclassified drug
dc.subjectanimal cell
dc.subjectcell culture
dc.subjectcell expansion
dc.subjectcell maturation
dc.subjectcell stimulation
dc.subjectcontrolled study
dc.subjectcumulus cell
dc.subjectdose time effect relation
dc.subjectfemale
dc.subjecthormone action
dc.subjectin vitro study
dc.subjectnonhuman
dc.subjectprotein expression
dc.subjectregulatory mechanism
dc.titleEffects of FSH on the expression of receptors for oocyte-secreted factors and members of the EGF-like family during in vitro maturation in cattle
dc.typeOtro


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