Bone morphogenetic protein 15 and fibroblast growth factor 10 enhance cumulus expansion, glucose uptake, and expression of genes in the ovulatory cascade during in vitro maturation of bovine cumulus-oocyte complexes

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCaixeta, Ester S.
dc.creatorSutton-McDowall, Melanie L.
dc.creatorGilchrist, Robert B.
dc.creatorThompson, Jeremy G.
dc.creatorPrice, Christopher A.
dc.creatorMachado, Mariana F.
dc.creatorLima, Paula F.
dc.creatorBuratini, José
dc.date2014-05-27T11:29:52Z
dc.date2016-10-25T18:50:45Z
dc.date2014-05-27T11:29:52Z
dc.date2016-10-25T18:50:45Z
dc.date2013-07-01
dc.date.accessioned2017-04-06T02:29:35Z
dc.date.available2017-04-06T02:29:35Z
dc.identifierReproduction, v. 146, n. 1, p. 27-35, 2013.
dc.identifier1470-1626
dc.identifier1741-7899
dc.identifierhttp://hdl.handle.net/11449/75828
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75828
dc.identifier10.1530/REP-13-0079
dc.identifierWOS:000321239900006
dc.identifier2-s2.0-84880529704
dc.identifierhttp://dx.doi.org/10.1530/REP-13-0079
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896560
dc.descriptionOocyte-secreted factors (OSFs) regulate differentiation of cumulus cells and are of pivotal relevance for fertility. Bone morphogenetic protein 15 (BMP15) and fibroblast growth factor 10 (FGF10) are OSFs and enhance oocyte competence by unknown mechanisms. We tested the hypothesis that BMP15 and FGF10, alone or combined in the maturation medium, enhance cumulus expansion and expression of genes in the preovulatory cascade and regulate glucose metabolism favouring hyaluronic acid production in bovine cumulus-oocyte complexes (COCs). BMP15 or FGF10 increased the percentage of fully expanded COCs, but the combination did not further stimulate it. BMP15 increased cumulus cell levels of mRNA encoding a disintegrin and metalloprotease 10 (ADAM10), ADAM17, amphiregulin (AREG), and epiregulin (EREG) at 12 h of culture and of prostaglandin (PG)-endoperoxide synthase 2 (PTGS2), pentraxin 3 (PTX3) and tumor necrosis factor alpha-induced protein 6 (TNFAIP6 (TSG6)) at 22 h of culture. FGF10 did not alter the expression of epidermal growth factor-like factors but enhanced the mRNA expression of PTGS2 at 4 h, PTX3 at 12 h, and TNFAIP6 at 22 h. FGF10 and BMP15 stimulated glucose consumption by cumulus cells but did not affect lactate production or levels of mRNA encoding glycolytic enzymes phosphofructokinase and lactate dehydrogenase A. Each growth factor increased mRNA encoding glucosamine:fructose-6-PO4 transaminases, key enzymes in the hexosamine pathway leading to hyaluronic acid production, and BMP15 also stimulated hyaluronan synthase 2 (HAS2) mRNA expression. This study provides evidence that BMP15 and FGF10 stimulate expansion of in vitro-matured bovine COCs by driving glucose metabolism toward hyaluronic acid production and controlling the expression of genes in the ovulatory cascade, the first acting upon ADAM10, ADAM17, AREG, and EREG and the second on downstream genes, particularly PTGS2. © 2013 Society for Reproduction and Fertility.
dc.languageeng
dc.relationReproduction
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject6 phosphofructokinase
dc.subjecta disintegrin and metalloprotease 17
dc.subjectADAM protein
dc.subjectADAM10 endopeptidase
dc.subjectamphiregulin
dc.subjectbone morphogenetic protein 15
dc.subjectepiregulin
dc.subjectfibroblast growth factor 10
dc.subjectglucose transporter 1
dc.subjectglucose transporter 4
dc.subjectglutamine fructose 6 phosphate aminotransferase
dc.subjectgrowth factor
dc.subjecthexosamine
dc.subjecthyaluronan synthase 2
dc.subjecthyaluronic acid
dc.subjectlactate dehydrogenase
dc.subjectlactic acid
dc.subjectmessenger RNA
dc.subjectpentraxin 3
dc.subjectprostaglandin endoperoxide
dc.subjectprostaglandin endoperoxide synthase 2
dc.subjecttumor necrosis factor alpha
dc.subjecttumor necrosis factor alpha induced protein 6
dc.subjectunclassified drug
dc.subjectanimal cell
dc.subjectcell maturation
dc.subjectcontrolled study
dc.subjectcow
dc.subjectcumulus cell
dc.subjectenzyme synthesis
dc.subjectgene expression
dc.subjectglucose intake
dc.subjectglucose metabolism
dc.subjectglucose transport
dc.subjectin vitro study
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectoocyte
dc.subjectovary
dc.subjectpriority journal
dc.subjectprotein expression
dc.titleBone morphogenetic protein 15 and fibroblast growth factor 10 enhance cumulus expansion, glucose uptake, and expression of genes in the ovulatory cascade during in vitro maturation of bovine cumulus-oocyte complexes
dc.typeOtro


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