SERS mapping in langmuir-blodgett films and single-molecule detection

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorAoki, Pedro H.B.
dc.creatorCarreon, Eduardo G.E.
dc.creatorVolpati, Diogo
dc.creatorShimabukuro, Milton H.
dc.creatorConstantino, Carlos J.L.
dc.creatorAroca, Ricardo F.
dc.creatorOliveira Jr., Osvaldo N.
dc.creatorPaulovich, Fernando V.
dc.date2014-05-27T11:29:04Z
dc.date2016-10-25T18:48:07Z
dc.date2014-05-27T11:29:04Z
dc.date2016-10-25T18:48:07Z
dc.date2013-05-01
dc.date.accessioned2017-04-06T02:22:46Z
dc.date.available2017-04-06T02:22:46Z
dc.identifierApplied Spectroscopy, v. 67, n. 5, p. 563-569, 2013.
dc.identifier0003-7028
dc.identifier1943-3530
dc.identifierhttp://hdl.handle.net/11449/75275
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75275
dc.identifier10.1366/12-06909
dc.identifierWOS:000318262700012
dc.identifier2-s2.0-84880049223
dc.identifierhttp://dx.doi.org/10.1366/12-06909
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896022
dc.descriptionPlasmon-enhanced spectroscopic techniques have expanded single-molecule detection (SMD) and are revolutionizing areas such as bio-imaging and single-cell manipulation. Surface-enhanced (resonance) Raman scattering (SERS or SERRS) combines high sensitivity with molecularfingerprint information at the single-molecule level. Spectra originating from single-molecule SERS experiments are rare events, which occur only if a single molecule is located in a hot-spot zone. In this spot, the molecule is selectively exposed to a significant enhancement associated with a high, local electromagnetic field in the plasmonic substrate. Here, we report an SMD study with an electrostatic approach in which a Langmuir film of a phospholipid with anionic polar head groups (PO 4 -) was doped with cationic methylene blue (MB), creating a homogeneous, two-dimensional distribution of dyes in the monolayer. The number of dyes in the probed area of the Langmuir-Blodgett (LB) film coating the Ag nanostructures established a regime in which single-molecule events were observed, with the identification based on direct matching of the observed spectrum at each point of the mapping with a reference spectrum for the MB molecule. In addition, advanced fitting techniques were tested with the data obtained from micro-Raman mapping, thus achieving real-time processing to extract the MB single-molecule spectra. © 2013 Society for Applied Spectroscopy.
dc.languageeng
dc.relationApplied Spectroscopy
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectSERS
dc.subjectSingle-molecule detection (SMD)
dc.subjectVisual data exploration
dc.subjectMicro-Raman mapping
dc.subjectRealtime processing
dc.subjectSingle molecule level
dc.subjectSingle-molecule detection
dc.subjectSpectroscopic technique
dc.subjectTwo dimensional distribution
dc.subjectAromatic compounds
dc.subjectElectromagnetic fields
dc.subjectMapping
dc.subjectMolecular biology
dc.subjectPhospholipids
dc.subjectPlasmons
dc.subjectSilver
dc.subjectSubstrates
dc.subjectMolecules
dc.titleSERS mapping in langmuir-blodgett films and single-molecule detection
dc.typeOtro


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